Objective: To compare the diagnostic yields of KOH mount and PAS staining of nail clippings for demonstration of hyphae
The "Paris'" type has intracellular hyphae
, which can be folded; "Arum" is observed in intercellular hyphae
and arbuscules; and "intermediary" has both "Arum" and "Paris" structures (DICKSON, 2004).
All these cases show positivity with GMS in the form of fungal hyphae
and spores and were negative for PAS.
This fungus with the help of their enzymes and toxic compounds damages its host and absorb food from the host cells by entering hyphae
to the host (Benitez et al, 2004; Vinale et al.
Phaeohyphomycosis is a collective term for a heterogeneous group of opportunistic fungal infections that contain dematiaceous yeast-like cells and hyphae
Abundant, branched pseudohyphae and true hyphae
with blastoconidia are present.
A KOH (potassium hydroxide) preparation will demonstrate hyphae
or blastopores in CCV.
The presence of necrotic masses and calcifications that easily detach, with white-yellowish coloration, whose dimensions vary from 2 to 10 millimeters in diameter, containing hyphae
and the infiltrate of eosinophils called "kunkers", with plates and fistules and fibrinous-bloody Discharge, are unmistakable signs of Pythiosis (6).
Histologically, multifocal necrotic, granuloma were seen with central radiating septated and branching hyphae
of Aspergillusfumigatusin the lungs and liver.
A focally extensive area of pericapsular exudation was present in the left kidney, with fungal hyphae
and infiltration of heterophils, lymphocytes, and macrophages through the capsule, extending into the subcapsular interstitium in the cortex.
Anatomic pathology identified necrotic bone with invasive fungal hyphae
In the case of Indian pipe, the plant taps into the abundant strands of fungal hyphae
(branching filaments) that form a dense and interwoven network in most forest soils.