Criteria included 1) clinical presentation with influenza-like signs and symptoms, pneumonia, meningitis, or involvement of bones, joints, or skin; and 2) laboratory confirmation with cultural, histopathologic, or molecular evidence of Coccidioides, or positive serologic test results for coccidioidal IgM (by immunodiffusion
, enzyme immunoassay [EIA], latex agglutination, or tube precipitin) or for coccidioidal IgG (by immunodiffusion
, EIA or complement fixation).
However, the serum Coccidioides IgM and IgG by EIA were both positive, as well as Coccidioides immunodiffusion
for antibodies, but Coccidioides antibody complement fixation and pleural fluid Coccidioides antigen were negative.
Contract notice: Reagents, tests and operating supplies for laboratory devices (hematological counter MEDONIC M 20 , two-channels coagulometer SIEMENS BFT II , one-channel aggregometer 591A, specific immunodiffusion
boards, device PHAST SISTEM , electrolyte analyzer AVL 9180 , coagulometer DIAMED-CD 4 , hematological analyzers SYSMEX KX21N and XT 1800i and immunological analyzer ARCHITECT I1000 sr ).
Antibody testing: Aspergilus IgG, and Histoplasma, Blastomyces, Coccidioides, & Aspergillus by Immunodiffusion
plates were prepared using 1% agarose.
IgA and IgG were determined by radial immunodiffusion
The serum IgG, IgM and IgA contents were determined by the radial immunodiffusion
technique according to Ishikawa and Nanjo (2009).
Layers inoculated with the LPAIV A/Bobwhite Quail/Pennsylvania/20304/98 (H7N2) were tested for antibody levels in the serum and egg yolk by using the agar gel immunodiffusion
test (AGID), hemagglutination-inhibition test (HI), anda commercially available enzyme-linked immunosorbent assay (ELISA).
Specificity of the rabbit antibody was tested using a double immunodiffusion
test (Ouchterlony & Nilsson 1973) and ELISA.
Serology for H capsulatum, obtained for the evaluation of fever of unknown origin, revealed mycelial and yeast complement fixation titers of 1:512 with a positive M and H band on immunodiffusion
10) Because of this and because more relevant tests, such as immunodiffusion
, immunoprecipitation, radioimmunoassay (RIA), hemagglutination, and enzyme immunoassay (EIA), for detecting specific autoantibodies to specific nuclear antigens are now widely available, interpretation of patterns has become much less important clinically and is being gradually replaced by these automated tests.
Agar Gel Immunodiffusion
(AGID) Test (after six months of age): Detects circulating antibodies in the blood.