indirect immunofluorescence


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Related to indirect immunofluorescence: Flow cytometry, ELISA, FITC, QPCR
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Noun1.indirect immunofluorescence - a method of using fluorescence microscopy to detect the presence of an antigen indirectly
fluorescence microscopy - light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes
References in periodicals archive ?
In this study, we aimed to expand our knowledge and understanding of bunyaviruses associated with febrile human illness in Peru, and these efforts focused on Maguari-like viruses, which were provisionally identified by using indirect immunofluorescence assays.
NOVA Lite DAPI ANA Kit is an indirect immunofluorescence assay for the qualitative detection and semi-quantitative determination of anti-nuclear antibodies of the IgG isotype in human serum by manual fluorescence microscopy or with NOVA View Automated Fluorescence Microscope.
Confirmatory indirect immunofluorescence was negative.
In all cases, the diagnosis was confirmed by direct and/or indirect immunofluorescence studies.
Detection of Em A using indirect immunofluorescence is considered the reference standard for CD-specific antibodies due to its unsurpassed sensitivity and specificity.
Serum antisperm antibodies were detected by indirect immunofluorescence test (EURO IMMUNE, Germany).
Indirect immunofluorescence assay (IFA) technique: IFA was carried out with antiserum raised in adult mouse as described earlier (5).
Immunostaining was performed using an indirect immunofluorescence method, which has been described in detail previously [Morgan et al.
5) The indirect immunofluorescence technique that is being used to determine C-ANCA titer is prone to false positives due to antibodies targeted to similarly distributed epitopes in the cytoplasm.
For the detection of C4d, indirect immunofluorescence was employed, using biotinylated mouse anti-human monoclonal antibody and a FITC-conjugated rabbit anti-mouse IgG antibody (Biogenesis, Poole, England).
The samples were analyzed for antinuclear antibodies (ANAs) using a commercially available indirect immunofluorescence test.
We then employed indirect immunofluorescence microscopy to examine localization of galectin-3 on HT-29,MSC-1 ,ASC-17D and MA-10 cells.