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intracerebral |
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| Members of phylogroup I are reported to be pathogenic for mice when introduced intramuscularly and intracerebrally. To isolate TBEV strains from the RT-PCR-positive tick pools, 20 [micro]L of the supernatant of the pools diluted 1:1 in D-PBS-BSA was injected intracerebrally into suckling NMRI mice. Central nervous system specimens were taken after patients' deaths and underwent immunofluorescent assay and histopathologic examination for rabies antigens; also, specimens were injected intracerebrally into suckling mice in an attempt to isolate the virus. |
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