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isoelectric point |
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isoelectric point n (Life Sciences & Allied Applications / Biochemistry) Biochem the pH value at which the net electric charge of a molecule, such as a protein or amino acid, is zero How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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| Isoelectric focusing showed that AX476 produced a [beta]-lactamase with an isoelectric point of 7. Separation can be done simply at the whole-protein level using two-dimensional gel electrophoresis; this technique separates molecules by molecular weight and isoelectric point (the pH at which a molecule carries no net electrical charge). The mixed-mode sorbents can distinguish proteins that have similar or very close isoelectric points, a separation that cannot be achieved with a single ion exchange step. |
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