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isopropanol |
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Rotavirus double-stranded RNA (dsRNA) was extracted directly from stool by the TRIzol method (Invitrogen, Carlsbad, CA, USA) and precipitated with isopropanol. Sof-Wick cotton sponges (Johnson & Johnson, New Brunswick, NJ) were saturated with 10 mL isopropanol and wiped across a 1-[ft. The particle size distribution (PSD) of the solids dispersed in isopropanol was determined by gravitational and centrifugal sedimentation analysis (SA-CP3 particle size analyzer, Shimadzu Scientific Instruments, Inc. |
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