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isotype |
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| 3] at 4[degrees]C for 15 min with optimal concentrations of antibodies: a) memory helper T cells: CD4-FITC/CD45RO-PE; b) memory cytotoxic T cells: CD8-FITC/CD45RO-PE; c) activated cytotoxic T cells: CD8-FITC/HLADR-PE; d) activated T cells bearing the IL-2 receptor: CD3-FITC/CD25-PE; and e) isotypic controls IgG-FITC and IgG-PE. Mouse IgM preimmune serum was used at the same concentration as the primary antibody to provide an isotypic control. Continued progress in identifying isotypic selective HDAC inhibitors in inflammation, CNS diseases, diabetes, and fungal infections. |
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