Mouse monoclonal antiactive [beta]-catenin (ABC) which is specific against nuclear [beta]-catenin, and goat anti-mouse IgG-rhodamine were obtained from Millipore (Massachusetts, USA), whereas rabbit polyclonal anti-cleaved caspase-3 antibody was from Calbiochem (Massachusetts, USA), EMD Chemicals, goat anti-rabbit IgG-FITC, rabbit anti-goat IgGHRP conjugated and IgG1 mouse isotypic
control were from Vector Laboratories (Peterborough, UK).
FACopyrightrey, Different Adsorption Behaviors of Methane and Carbon Dioxide in the Isotypic
Nanoporous Metal Terephthalates MIL-53 and MIL-47, J.
Cells were washed twice by phosphate buffered solution (PBS) and then incubated with either isotypic
control or monoclonal antibodies against PE-CD45, FITC-CD71, FITC-CD117, APC-CD115, and APC-RANKL (receptor activator of nuclear factor [kappa]B ligand) for 30 min on ice in the dark, respectively.
This is not the case of planar systems; on the contrary, it simplifies the analysis because of the reduction to isotypic
Studies on Plasmodium falciparum isotypic
antibodies and numbers of IL-4 and IFNgamma secreting cells in paired maternal cord blood from South West Cameroon.
space U(r,[mu]) inherits the action of [G.
We used isotypic
controls in order to check for unspecific binding and to set the markers during the evaluation of the results.
The (canonical) isotypic
decomposition of V is the direct sum of its kG-homogeneous components.
antibody response to plaque anaerobes in periodontal disease.
Exudate fluids were immunoprecipitated with antimouse IgG Dynal[R] magnetic beads (Invitrogen) with, as capture antibodies, 5A6G7, 01G, MEM-G/9 or G233 anti-HLA-G antibodies, W6/32 anti-pan-HLA-I antibody, or an unspecific isotypic
The mean fluorescence intensity (MFI) of the cells stained with isotypic
control antibodies was subtracted from the MFI of the cells stained with specific antibodies to get a measure of surface receptor-specific MFI.
Each specimen's event clusters were considered positive or negative when compared with the degree of the same specimen stained with the isotypic