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| Beginning in October 2003, Salmonella gene amplification (BAX System PCR Assay, DuPont Qualicon, Wilmington, DE, USA) was performed on lysed cells after overnight incubation in buffered peptone broth (35[degrees]C). The adhesions were lysed, the mucosa was scraped off the footplate, and the area of the annulus was denuded. Frozen tissue from three pups in each pool was lysed with 4 volumes of 9. |
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