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Unlike Salmonella, however, Shigella rapidly lyses the surrounding vacuole and is released into the cytosol, where it grows and divides (45). FTA is a chemically-treated matrix that lyses cells, denatures proteins and protects nucleic acid from nucleases, oxidation and UV damage. Methanol lyses the cells, solubilizes the cyclosporine, and precipitates most of the blood proteins. |
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