The aim of the present study was to investigate the release of polar bodies and monitor meiotic
development, including the most frequent number of chromosomes in Anomalocardia brasiliana, to establish a basis for future chromosome manipulation for aquaculture purposes.
The presence of sustentacular cells with high amounts of Follicle stimulating hormone (FSH) and testosterone progressed meiotic
division, reduced apoptosis and enhanced survival of germ cells in cultured human testis tissue or cell suspensions (Tesarik et al.
According to previous reports (25,29), meiotic
progress, oocyte growth, cumulus cell proliferation, and numerous other processes associated with IVM are stimulated by the above mentioned growth factors and cytokines.
Previously, we showed that the immunoreactive Egr3 colocalizes with meiotic
spindle and accumulates near cytosolic microtubule organizing centers (MTOCs) in oocytes during meiotic
05) was observed in meiotic
resumption and nuclear maturation rate in all three types of maturation media.
Accurate chromosome segregation requires a correctly assembled meiotic
spindle and so it is essential to understand this process.
At the age of 22-23 days, the most advanced germ cells are late spermatocytes (Clermont, 1972), allowing study of the whole meiotic
phase under our culture conditions (Staub et al.
Extra chromosome is often maternal and occurs because of meiotic
The 24 articles in this volume discuss various aspects of recombinational DNA repair, meiotic
recombination, and the regulation of these processes.
For example, researchers must show evidence of the correct nuclear DNA content at specific meiotic
stages, normal chromosome number and organization, and the capacity of germ cells to produce viable offspring.
Oocyte maturation, which can be simulated in in vitro conditions, deserves particular attention because meiotic
division and achievement of developmental competence are finalized during this short and extremely important period (summarized in ).
However, a genetic programme intrinsic to germ cells probably controls the progressive steps through mitotic, meiotic
and post- meiotic
phases of development.