5 x 10[sup]9/L with 3% promyelocyte, 15% myelocyte
, 19% metamyelocytes, 48% neutrophils, 4% lymphocytes, and 11% monocytes; and platelet count 81 x 10[sup]9/L.
Immature neutrophils include promyelocyte myelocyte
, metamyelocyte and band form.
Severe congenital neutropenia appeared unlikely as the bone marrow did not show 'maturation arrest' at the myelocyte
stage of development, the promyelocytes were not increased nor showed dysplastic morphology such as large size, atypical nuclei or vacuolated cytoplasm.
The predominant cell type is eosinophil myelocyte
, but there is a spectrum of forms including eosinophil metamyelocytes, eosinophils bands, and mature eosinophils.
The cells do not divide after the stage of myelocyte
16 Immune cells were identified and grouped as either myelocyte
cells or lymphoid cells based on morphology as observed via microscopy using H and E stained slides.
9 Platelets * * * Mean Platelet Volume * * * Neutrophils 7 29 58 Bands 54 50 35 Lymphocytes 10 9 5 Monocytes 7 0 2 Metamyelocyte 12 9 0 Myelocyte
8 2 0 Promyelocyte 2 1 0 Nucleated Red Blood Cells 2 0 2 Polychromasia present [dagger] [dagger] Poikilocytosis present [dagger] present Anisocytosis present [dagger] present Microcytes present [dagger] [dagger] Spherocytes present [dagger] [dagger] Mega-thrombocytes present present [dagger] Absolute Neutrophils 3.
We also measured and compared the level of N6AMT1 mRNA in several cell lines, including 293 (human embryonic kidney cells), HeLa, UROtsa, and HL60 (human pro myelocyte
Most eosinophil precursors were in the myelocyte
stage, but there also were a few metamyelocytes and rod-shape eosinophils.
Tissue examination showed granulocytic cells in different stages of maturation, but with a distinct and prominent shift toward immature forms at the promyelocyte and myelocyte
Bone marrow biopsy proved pronounced synchronisation, with virtually no maturation beyond the myelocyte
stage and marrow hypoplasia.
In the aspiration smear, 18% myelocyte
, 35% metamyelocyte, 11% polymorphonuclear leukocyte, 28% lymphocyte, 2% normoblast, 1% monocyte, 1% eosinophil, and 4% lymphoblast-like cells were present.