These include techniques based on molecular imaging, advanced genomic and transcriptomic methods of detection of clinically significant biomarkers and identification of nucleosomal
Similarly, a recent study in our laboratory indicated that garlic compounds generate MDA production in HL-60 cells leading to phosphatidylserine externalization, caspase-3 activation, nucleosomal
DNA fragmentation, and cell death (Yedjou and Tchounwou, 2012).
Circulating fragmented nucleosomal
DNA and caspase-3 mRNA in patients with lymphoma and myeloma.
protein, HMGB1, has recently been established as a late mediator of lethal systemic inflammation with a relatively wider therapeutic window for pharmacological interventions.
Mechanisms inducing sperm DNA damage: During sperm nuclear condensation, a dramatic sequence of events occurs involving topological rearrangements, transition of DNA-binding proteins, alterations in transcription and loss of nucleosomal
A positive role for histone acetylation in transcription factor access to nucleosomal
Such a pattern corresponded to inter nucleosomal
cleavage, which was characteristic of apoptosis.
DNA was extracted according to the protocol provided with the apoptosis kit, (G.
Acetylation of the e amino lysines weakens the electrostatic attraction for the (negatively charged) nucleic acids and disassembles the nucleosomal
histones, further the DNA sequence is accessible for various transcriptional factors and activates the gene transcription whereas deacetylation results in chromatin condensation and gene repression (30,31).
Formation of the nucleosomal
DNA ladder is the hallmark of apoptosis so analysis of the pattern of DNA isolated from the cells exposed to different stress inducers was done.
DNA in plasma resulting from apoptotic death of the tumor cells may also provide an indication for tumor DNA.
In some cancer cells, there is an overexpression of HDACs, or an aberrant recruitment of HDACs to oncogenic transcription factors causing hypoacetylation of core nucleosomal