Commonly known as the OLA, the Oligonucleotide Ligation Assay
is an analyte specific reagent (ASR) that is centered around the hybridization of a PCR primer with an exact match to a target sequence.
The systems we evaluated included (a) CF oligonucleotide ligation assay
Mutation in the gene coding for coagulation factor V and resistance to activated protein C: detection of the genetic mutation by oligonucleotide ligation assay
using a semi-automated system.
It will complement other technologies from the company including the Polymerase Chain Reaction (PCR) and Oligonucleotide Ligation Assay
Other publications demonstrated that the oligonucleotide ligation assay
(36) and single base chain extension (32) could be used for genotyping DNA samples with the microbead approach.
Rapid prenatal diagnosis of sickle cell diseases using oligonucleotide ligation assay
coupled with laser-induced capillary fluorescence detection.
Testing the feasibility of DNA typing for human identification by PCR and an oligonucleotide ligation assay
The oligonucleotide ligation assay
(OLA) relies on hybridization with specific oligonucleotide probes that can effectively discriminate between the wild-type and variant sequences.
Minor] mutations from a large number of samples, we utilized PCR followed by oligonucleotide ligation assay
These ambiguities can be circumvented by the use of genotyping methods such as allele-specific oligonucleotide hybridization (9), restriction enzyme analysis (ASRA) (10,11), the amplification refractory mutation system (12), oligonucleotide ligation assay
(13), heteroduplex analysis (14), or single-strand conformation polymorphism (15).