After incubation, serum opsonized
zymosan (25 uL) and intracellular reactive oxygen species detecting probe (25 uL), luminol were added into each well, except blank wells containing only HBSS++.
The possibility for clearing a higher number of opsonized
bacteria by phagocytes was markedly outlined at the background of reduced phagocytosis.
Literature suggests that antibody detection by elution is as efficacious as performing flow cytometry for the detection of antibody from a small population of opsonized
3-Aminophthalhydrazide (luminol), concanavalin A (Con A), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoIium bromide (MTT) reagent, fluorescein isothiocyanate (FITC)-labeled opsonized
Escherichia coli, fetal bovine serum (FBS), ficoll, Hank's balance salt solution (HBSS), lipopolysaccharide (LPS), Lymphoprep[TM] gradient (1077 mg/ml), phosphate-buffered saline (PBS) tablet, phorbol 12-myristate 13-acetate (PMA), Roswell Park Memorial Institute (RPMI)-1640, streptomycin, penicillin and trypan blue reagent were purchased from Sigma-Aldrich Corp (St Louis, MO, USA).
After contact with opsonized
microorganism, phagocytosis occurs.
Binding of receptors for the Fc portion of antibodies (Fc receptors) to opsonized
microorganisms is one of the most important mechanisms for pathogen recognition and activation of neutrophils .
Recently, we showed that neutrophils efficiently phagocytose and kill opsonized
NTHi bacteria (29), but decreased neutrophil phagocytic capacity among the elderly may impair this host defense and contribute to poorer clinical outcomes during NTHi infection.
The complement system triggers a biochemical cascade in which foreign cells are first opsonized
zymosan was separated by centrifugation, washed three times and suspended in HBSS.
The receptor also contributes to phagocytosis of opsonized
The phagocytic capability of adherent macrophages was dogged by using 3% suspension of unopsonized and opsonized
SRBC inoculation into the culture medium for the study of engulfment percentage and engulfment/cell competence of macrophages.
Nowday, carrier vehicles for the delivery of hydrophobic drugs are associated with several disadvantages: conventional emulsions, micelles and liposomes are thermodynamically unstable; lipophilic carriers cluster in blood flow and are rapidly opsonized
and massively cleared by liver and spleen; loading capacity of hydrophobic drugs into hydrophilic carriers is limited.