As a natural non-specific defense factor in humans and animals, lysozyme can destroy the cell walls of Gram positive bacteria by hydrolyzing the link of [beta]-1,4 glycosidic bonds between the peptidoglycan
N-acetyl glucosamine and the N-acetylmuramic acid, thus damaging the bracket of the peptidoglycan
causing the cells to disintegrate by internal osmotic pressure (Irwin, 2004; Corin et al.
Gram-positive bacteria have a thick layer of peptidoglycan
protecting the cell membrane, whereas gram-negative bacteria have an exposed cell membrane displaying lipopolysaccaride (LPS), a carbohydrate-fat complex.
For cell wall analysis, extraction and separation of peptidoglycan
precursors was performed as described (7).
It is known that the component of Gram-positive bacteria cell walls is peptidoglycan
, which confers the hydrophobic character of S.
Instead, the dye gets trapped in a tight package of sugar-filled polymers, called peptidoglycan
, which envelops bacterial cells.
The main component of the wall is called peptidoglycan
, which is the key target of penicillin and other similar antibiotics.
Objective: "In this project, I will investigate the proteins involved, and their interactions, in the formation of both peptidoglycan
(PG) synthesis core complexes (elongasome and septosome) in the model bacterium Escherichia coli to gain new molecular insights into how the cell grows its cell wall during the cell cycle.
This reaction is enhanced by soluble peptidoglycan
(PGN) up to a concentration of 10 pg/ml, resulting in a reduction of the limit of detection for LPS by a factor of 10.
is present from bacteria in platelets, it triggers a reaction cascade which ends in the development of a red color.
This work zeroes in on trying to stop construction of a bacterial cell layer called peptidoglycan
, a mesh-like structure that, in gram-negative bacteria like E.
Bactericidal action is achieved by interference of peptidoglycan
biosynthesis: an early stage of bacterial cell wall development (13).
The periplasmic space contained fine filamentous material, which probably represent peptidoglycan