Different phenotypic and genotypic methods have been used to subtype Salmonella Enteritidis, including techniques such as phage typing
and pulsed-field gel electrophoresis (PFGE).
Antibiogram, phage typing
& biotyping of Salmonella Typhi & Salmonella Paratyphi A from Rourkela, Orissa.
Comparison of Multiplelocus variable-number tandem repeat analysis, pulsedfield gel electrophoresis, and phage typing
for subtype analysis of Salmonella enterica serotype Enteritidis.
Because the isolates produced an uninterpretable pattern by pulsed-field gel electrophoresis, selected isolates were further characterized by phage typing
at the National Laboratory for Enteric Pathogens, Laboratory Center for Disease Control, in Ottawa (Ontario, Canada).
Our analyses of the serotype and phage typing
dataset enabled us to examine the size and duration of epidemics of foodborne infection caused by subtypes belonging to a range of serovars of S.
cholerae were sent to National Institute of Cholera and Enteric Diseases (NICED), Kolkata, for confirmation and phage typing
Results of phage typing
by using standard techniques (7) were available for 74 isolates; 54 were classified as phage type Dundee, 19 as a uniform "reactions do not conform," which closely resembled Dundee, and 1 as untypeable.
was done at Vibrio phage reference laboratory National Institute of Cholera and Enteric Disease (NICED), Kolkata, following the conventional Basu and Mukherjee's method and the new phagetyping scheme (12,13).
Isolate subtyping was conducted by phage typing
, multilocus variable number tandem repeat analysis (MLVA), multilocus sequence typing (MLST), and XbaI-PFGE (9,10) (http://mlst.
We carried out investigation of two cholera outbreaks, for identification, antimicrobial susceptibility testing, phage typing
and molecular characterization of isolated Vibrio cholerae O1, and to suggest prevention and control measures.
The Vi phage typing
scheme from the Colindale Institute London was used for Salmonella spp.
: Few select K cholerae O1 isolates were sent for confirmation to the National Institute of Cholera and Enteric Diseases (NICED), Kolkata, where phage typing
was done by the Vibrio Phage Reference Laboratory as per procedures published earlier.