phalloidin


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phalloidin

(fəˈlɔɪdɪn)
n
(Elements & Compounds) a peptide toxin, responsible for the toxicity of the death cap mushroom, Amanita phalloides
[C20: New Latin, from phallus + -oid + -in]
References in periodicals archive ?
The DPBS solution containing Rhodamine phalloidin (Thermo Fisher Scientific, Waltham, MA) was added for 15 minutes.
We used fluorescently labeled phalloidin and the pan neural stains synaptotagmin B and tyrosinated [alpha]-tubulin to investigate differences in the musculature between E.
Phalloidin staining of actin filaments in the body wall muscle cells disclosed no morphological abcrrancies, despite clearly impaired muscle cell function.
4, p75) Mouse Monoclonal mouse IgG1 Nestin Monoclonal Mouse IgG1 clone #196908 mouse IgG1 Pax6 Purified anti-Pax-6 antibody rabbit GFAP Anti-GFAP antibody [2A5] mouse IgG1 F-actin Alexa Fluor[R] 568 Phalloidin -- Target name Dilution Catalog # Provider HNK1 1:200 C6680 Sigma p75 1:200 #AB-N07 Advanced Nestin 1:500 MAB1259 R&D Systems Pax6 1:200 PRB-278P Covance GFAP 1:1000 ab4648 Abcam F-actin 1:1000 A12380 Invitrogen Tab.
The formation of F-actin stress fibers was assessed via immunostaining with phalloidin and examined using confocal microscopy.
Phalloidin conjugated with a derivative of rodamin was used to stain the filamentous actin (F-actin).
Cells were incubated with (1) serum free RPMI medium, (2) Alexa 546 dye, and (3) Alexa Fluor 546 labeled SHBG (red) in serum-free medium for 1 hour and then costained with Alexa Fluor 488 phalloidin (green) to outline the actin cytoskeleton and DAPI (blue) to localise nuclei.
1% TritonX-100 in PBS for 3 to 5 min and stained with Rhodamine Phalloidin (100 nM, Cytoskeleton) for 20 min.
4], galactosamine (Galc), and phalloidin and strongly inhibited the activity of 5-lipoxygenase in porcine leukocytes.
4]C1 (ammonium chloride), 5% FBS (fetal bovine serum), 2% goat serum and then incubated with fluorochrome-tagged phalloidin (Molecular Probes, Invitrogen; 1:200) and antibody C3-33 (1:100), which recognizes all three RyR isoforms at that dilution (Meissner 2002).
For actin filament visualization, the blocking solution was removed and cells were incubated with 200 il rhodamine phalloidin solution (0.
Cells were fixed and stained for polymerized actin with Rhodamine Phalloidin as described in (14c).