Micrographs were made in a photomicroscope
(BX-51, Olympus Optical Co.
Histological sections analysis was conducted on a Leica DM 750 photomicroscope
, with built-in digital camera ICC50 (HD-521420221).
Images capture were performed on the Zeiss AxioCam ERc 5s photomicroscope
on a 40X objective using the Image-Pro[R] Plus--Version 4.
Stained sections were evaluated with an Olympus BX51 photomicroscope
(Tokyo, Japan) and photographed with an Olympus DP72 digital camera (Tokyo, Japan).
Observations and imaging were performed using a photomicroscope
(AX70TRF, Olympus Optical, Tokyo, Japan) equipped with a U-Photo camera system.
A Zeiss Axioplan 2 Fluorescence Photomicroscope
(Carl Zeiss, Oberkochen, Germany) was used to observe the spermatocyte nuclei.
Conventional coloration with Giemsa 5% was used to observe diploid (2n) and fundamental (FN, excluding sexual chromosomes) numbers and chromosome morphology variation; 50 metaphases of each specimen were analyzed with a Nikon Eclipse 50i photomicroscope
under 1000 x magnification.
Sections were evaluated histologically using a light microscope (Olympus, CX23) and photomicrographs were taken using a photomicroscope
(Olympus, CX41) at a magnification of x200.
The microscopic photodocumentation was performed in a photomicroscope
Species identification was performed on permanent slides prepared with Naphrax[R] resin and with oxidized samples (Moreira-Filho and Valente-Moreira, 1981), and images were collected with the aid of an Olympus BX70 photomicroscope
with a coupled DP 071 camera.
Some slides were photomicrographed under an Olympus Vanox photomicroscope
with Kodak Ultra 400 film.
The images were obtained with a photomicroscope
(BX51, Olympus, Tokyo, Japan) coupled to a Sony camera using the software Image Pro-plus, v.