polymerase chain reaction

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Related to polymerase chain reactions: PCR amplification

polymerase chain reaction

n. Abbr. PCR
A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA as many as one billion times and is important in biotechnology, forensics, medicine, and genetic research.

pol′ymerase chain′ reac`tion

the laboratory production of numerous copies of a gene by separating the two strands of the DNA containing the gene segment, marking its location with a primer, and using a DNA polymerase to assemble a copy alongside each segment and continuously copy the copies. Abbr.: PCR
amplification en chaîne par polymérase
References in periodicals archive ?
Polymerase chain reactions (PCRs) were carried out in 25 [micro]L of reaction mixtures buffered with 20 mmol/L Tris-HC1 (pH 8.
Molecular diagnostic assays, such as nested polymerase chain reaction and a species-specific DNA probe from the ribosomal DNA complex, have been useful to identify P.
The construction technique used was splicing by overlap extension (SOE), which involves generating the two fragments of interest in separate polymerase chain reactions (PCR).

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