Canadian Forest Service (Canada) has patented an isolated polynucleotide
sequence useful as a promoter in plant root tissue.
7,273,718, entitled "Isolated Genomic Polynucleotide
Fragments from Chromosome 10q25.
Promoter sequences of the invention provide for seed-specific transcription of operably linked polynucleotide
35 cycles of denaturation at 94degC for 40 s, annealing at 52degC (for the nested-PCR) or 55degC (standard PCR) for 40 s, and polynucleotide
chain elongation at 72degC for 40s, and final extension at 72degC for 5 min.
Amplifying the target nucleotide sequence of the polyketide synthetase gene to produce an amplified polynucleotide
; detecting the presence of the amplified polynucleotide
by contacting the amplified polynucleotide
with a labeled molecular beacon; for determining the virulence of red tide blooms.
Lucigen's products include pSMART GC VectorspGC Blue VectorsBigEasy Long PCRCloneSmart, BigEasy Linear, DNATerminator End Repair Kit, CloneDirect Rapid Ligation Kit, UltraClone DNA Ligation and Transformation Kits, Gel-Ready DNA Ladders, NxGen T4 DNA Ligase, NxGen T4 RNA Ligase, NxGen Exonuclease I, NxGen Lambda Exonuclease, NxGen Exonuclease III, NxGen RNAse Inhibitor, RNase I, T4 Polynucleotide
Kinase, and Pyrophosphatase, among others.
35 cycles of denaturation at 94C for 40 s, annealing at 52C (for the nested-PCR) or 55C (standard PCR) for 40 s, and polynucleotide
chain elongation at 72C for 40s, and final extension at 72C for 5 min.
Researchers found that Tcl1 does its job by suppressing mitochondrial polynucleotide
phosphorylase, thereby inhibiting mitochondrial growth and metabolism.
Members of the AID/APOBEC family of enzymes deaminate cytosine (C) to uracil (U) in the context of a polynucleotide
Patent Application Number 13/336,028 entitled, "In Vivo Polynucleotide
Delivery Conjugates Having Enzyme Sensitive Linkages.
Additionally, DNA polynucleotide
fragments are also patentable subject matter in both the U.
The 5' terminus of full-length DNA was phosphorylated with 20 units of T4 polynucleotide
kinase and 0.