5 [micro]g/mL puromycin
for 3days to obtain stable transduction.
The individual stably transfected colony was subsequently selected in the presence of puromycin
Then AsT cells were transduced with virus, followed by the puromycin
The initial comparison of protocols used by participating laboratories revealed that cycloheximide or puromycin
was sometimes used for NMD treatment, with incubation times between 4 and 8 h and concentrations between 100 and 250 [micro]g/mL, the use of 8 cDNA synthesis kits, 12 different DNA polymerases, and transcript isolation strategies that included band excision, subcloning, and sequencing.
Selection of infected cells was done in DMEM containing 5 [micro]g/ml puromycin
for 24 hours after lipofection had been conducted for 24 hours.
Rats treated with puromycin
(nephritic syndrome model) caused increased proteinuria, podocyte foot process retraction, actin cytoskeletal rearrangement, and deranged nephrin distribution.
Mitochondrial dysfunction in focal segmental glomerulosclerosis of puromycin
The cells carrying the transgene were selected in medium containing 1 mg/ml puromycin
(Gibco BRL) for 5 days.
To address the hypothesis that AHR activation by TCDD during embryonic development disrupts expression of genes critical to cardiac differentiation, we generated an AHR-positive embryonic stem cell lineage that expresses puromycin
resistance and enhanced green fluorescent protein (eGFP) under the control of the AHR-responsive Cyplal promoter.
Divergent effects of low versus high dose anti-TGF-beta antibody in puromycin
aminonucleoside nephropathy in rats.
Stocks of FAO-PPAR[alpha] cells were maintained in DMEM with 5% fetal bovine serum (FBS) and 2 [micro]g/mL puromycin
(both from Sigma-Aldrich).
2] ratio in rats with puromycin
aminonucleo-side-induced nephrosis (Nagasawa et al.