Calcineurin inhibitors cyclosporin A and tacrolimus protect against podocyte injury induced by puromycin
aminonucleoside in rodent models.
5x 105 cells/well) and puromycin
selection (1 [micro]g/mL) was applied at 24h post-seeding.
After 48 h, transfectants were diluted in 1:10 ratio, and the antibiotic selection was performed in a medium containing 5 [micro]g/ml puromycin
(Life Technologies, USA) for 4 weeks.
selection was performed in the presence of 2 mg/ml puromycin
for 2 weeks.
After infection, the cells were selected with 3 [micro]g/mL puromycin
(Enzo Life Sciences, Farmingdale, NY) for 3 days.
The strand also has a puromycin
selection gene for enriching cell populations that uptake the RNA strand, increasing reprograming efficiencies and success.
5 [micro]g/mL puromycin
for 3days to obtain stable transduction.
After lentivirus infection and puromycin
selection, the drug-resistant cells were obtained.
A laboratory at Harvard Medical School chemically modified the antibiotic puromycin
in a way that made it possible to visualize and quantify the amount of protein synthesized by individual cells within the body.
Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin
13: Bioisosteric relationship between puromycin
The initial comparison of protocols used by participating laboratories revealed that cycloheximide or puromycin
was sometimes used for NMD treatment, with incubation times between 4 and 8 h and concentrations between 100 and 250 [micro]g/mL, the use of 8 cDNA synthesis kits, 12 different DNA polymerases, and transcript isolation strategies that included band excision, subcloning, and sequencing.