Treatment with VOO reduced the changes, so that only focal nuclear pyknosis
and cytoplasmic vacuolation were detected in the DM plus VOO group (figure 3B).
In hepatopancreatic cells of the diseased crab (Figure 2), condensation and margination of nuclear chromatin, disappearance and deformation of mitochondria cristae, and endoplasmic reticulum and lysosomes disintegration, and different size of vesicles could be observed Nuclear condensation, cell atrophy and intercellular space and a lot of spermatogonium with distinct nuclear pyknosis
and vesicles could be observed in gonad of the diseased crabs the membrane of spermatogonium was thicken and rucked up (Figure 3).
Features of follicle atresia and cystic formations were described (Figure 1): cell pyknosis
, interruption of the basement membrane and thecal layers' disruption, reduction of granulosa cells layers, presence of elongated epithelioid cells in the surface lining the follicle wall.
Cellular debris of necrotic beta cells appeared as eosinophilic smudges and necrotic changes in nuclei like pyknosis
, karyolysis and karyorrhexis were observed.
The acinar changes typically associated with radiation therapy or hormone/androgen-deprivation therapy, such as acinar atrophy, cytoplasmic vacuolization, nuclear pyknosis
, and basal cell hyperplasia, are not usually seen in the postcryotherapy setting.
In contrast, most neurons in the cortical lesions of the vehicle-treated mice exhibited some or all of the following features: Nissl bodies' reduction, chromatolysis, nuclear pyknosis
, eosinophilic cytoplasm, or a lack of cellular structure.
Figure 2d showed a mouse treated with acetaminophen with extensive centrilobular necrosis, hydropic degeneration of hepatocytes, destruction of central vein endothelium, karyolysis, pyknosis
, and karyorrhexis of nuclei.
It was observed that administration of 156 mg/kg and 312 mg/kg of the extract caused pyknosis
of the nuclei.
of nuclei less and sullaphing in central vein.
The apoptotic cells exhibited nuclear chromatin condensation and dense staining gathered at the nuclear membrane with pyknosis
or fragmentation of apoptotic bodies (Fig.
The liver of Labeo rohita showed marked histopathological changes, including degeneration and necrosis of the hepatocytes, vacuolar degeneration in the hepatocytes, thrombosis formation in central veins, dilation, hypertrophy and pyknosis
of a nucleus in the hepatic tissues, irregular shaped nucleus and cytoplasmic vacuolation.
Several skeletal muscles showed polyphasic segmental necrosis (Figure 2) characterized by enlarged eosinophilic fibers with loss of cross striations, and nuclear pyknosis