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The 16S rRNA gene sequence of the isolate, named as strain KP10, had been submitted in GenBank and accessioned as KX013388.
A built-in multi-channel potentiostat reads the electrical current from the steady-state enzymatic cycling amplification: signal is proportional to the bound 16S rRNA content from lysate and reported in ranges of colony forming units (CFU) per milliliter through an established calibration curve.
The 16S rRNA genes have been used as the marker gene to determine the composition of both bacterial and archaeal communities because it is phylogenetically conserved and it does not laterally transfer .
The present research work is based on the molecular study of Ropalida brevita species from the genus Ropalida of the Subfamily Polistinae of family Vespidae using 12S rRNA gene and Cytochrome 1 (Co1) gene of collected from Abbottabad, Pakistan.
We amplified the hypervariable region V3-V4 of the 16S rRNA (422 bp) using PCR primers (S-D-Bact-0341-b-S-17/SD-Bact-0785-a-A-21) (9), following a modified 16S MiSeq protocol and barcoded samples before sequencing on a MiSeq (Illumina, Victoria, British Columbia, Canada).
The detection was done by biochemical screening assays and phylogenetic of 16S rRNA gene sequences based diagnostic polymerase chain reaction (PCR) for molecular identification.
En los arboles filogeneticos moleculares inferidos de los genes COII y 16S rRNA, todas las poblaciones examinadas de las 2 especies se agruparon en un clado comun con un alto valor de bootstrap.
8S gene were amplified using the two primers, ITS-1 and ITS-4 which were synthesized on the basis of conserved regions of the eukaryotic rRNA gene reported by Zhang, Z.
Moreover, the organism was found genetically closer to Bacillus cereus than to Bacillus mycoides and Bacillus thuringiensis with respect to 16S rRNA gene sequence.
Multiple sequence alignment and phylogenetic analysis using 16S rRNA gene sequences of LSPQ-04253 (GenBank accession number KX024579) and those of C.
Fiona Stewart, NEB Next-Generation Sequencing product marketing manager, commented that the NEBNext rRNA Depletion Kit efficiently removes abundant RNAs, thus decreasing the number of sequencing runs needed and, therefore, the overall cost of sequencing.
Amplification and sequencing of the 16S rRNA: Because the biochemical identification of several isolates was not accurate, amplification and sequencing of the 16S rRNA for 29 and a control strain was performed to confirm bacterial identification.
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- RRM-containing coactivator activator/modulator