reading frame


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reading frame

n.
One of the three possible ways in which an mRNA sequence of nucleotides can be read as a series of base triplets to specify the amino acids in a protein chain.
Translations
cadre de lecture
References in periodicals archive ?
Duck hepatitis B virus expresses a regulatory HBx-like protein from a hidden open reading frame.
The expression vector of recombinant SPLUNC1 protein was constructed by inserting full length SPLUNC1 open reading frame (ORF) into pPIC9K plasmid (Invitrogen, China).
Upon further investigation, the team found that a tumor-suppressor gene, called alternative reading frame (ARF), which is normally suppressed in mouse and human iPSCs, remained active in the mole rat iPSCs.
Primers were designed to generate a product of easily sequenced length (around 650 bases) and be within the open reading frame of each locus greater than 100 base pairs from each end.
The research also indicated how these non-random results can be harnessed to produce a desired effect, such as a gene knockout or the reading frame restoration of a disease-causing allele.
Antisense oligonucleotide mediated exon skipping targets DMD pre-mRNA to induce skipping of specific exons and restore the open reading frame.
Scientists have identified a mutation in a gene called "chromosome 9 open reading frame 72" or C9ORF72.
The Comp16495 contig (GenBank KF717090) was 2,097 bp long, with an open reading frame encoding 680 amino acids.
DNA sequencing showed an open reading frame, 1476 bp in length, encoding a 492 amino acid polypeptide.
Skipping of exon 8 led to a frame-shift in coding sequences with a stop codon at position 372, [sup][3] which resulted in changes of 41 amino acids (residuals 331-371) and in-frame deletion of 126 amino acids in the reading frame.
Depending on the fact that mutations maintain or not of the reading frame, dystrophin will or not be present according to the Monaco rule [6].
In milder BMD, the reading frame remains intact (IN-frame), though a coding segment of the gene has been lost or reduced.