restriction endonuclease


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Noun1.restriction endonuclease - any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
endonuclease - a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
References in periodicals archive ?
At 10 and 24 hpf, gDNA extracted from TDCPP-treated embryos was incompletely digested by all methylation-sensitive restriction endonucleases tested, and banding patterns were similar to those of vehicle-treated controls (Figure 6).
In recent years, cytogenetic studies in bivalves have expanded as a result of the introduction of new molecular cytogenetic techniques such as in situ restriction endonuclease (RE) banding and fluorescence in situ hybridization (FISH).
Diagnosis of partially treated culture-negative bacterial meningitis using 16S rRNA universal primers and restriction endonuclease digestion.
35) Danna, K and D Nathans, 'Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae', Proceedings of the National Academy of Sciences USA, vol.
Use of low-frequency-clevage restriction endonucleases for DNA analysis in epidemiological investigations of nosocomial bacterial infections.
9,10) Genomic DNA was cut with a restriction endonuclease, and the resultant fragments were size-fractionated in an agarose gel and transferred to a nylon membrane for probing with locus-specific probes.
The plugs were then washed four times with TE buffer, washed twice with the appropriate restriction endonuclease buffer, and incubated overnight with the appropriate restriction enzyme.
The Stx-2A bacteriophages were highly similar in morphologic features, restriction endonuclease profiles, chromosomal integration sites, and superinfection immunity (2,3) and showed <65% similarity to Stx phages from non-O104 strains.
A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data.
include the use of a single-tube reaction that combines a rapid methylation-sensitive restriction endonuclease digestion and a quantitative PCR (qPCR), thus decreasing both hands-on time and possible cross-contamination.

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