restriction endonuclease


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Noun1.restriction endonuclease - any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology
endonuclease - a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes
References in periodicals archive ?
Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites.
Plasmid DNA was extracted & positive clones were identified by Colony PCR and restriction endonuclease analysis.
2%) with isolates available for restriction endonuclease analysis typing, 133 (38.
Amplicon size, restriction endonuclease and fragment lengths Site Amplicon Restriction Fragment lengths (bp) size endonuclease V249I 311 ACLI 107-204 V/V 107-204-311 V/I 311I/I T280M 311 BSMBI 75-118T/T 75-118-193 T/M 118-193 M/M TABLE 2.
Also, restriction analysis was performed using appropriate restriction endonuclease enzymes.
At 10 and 24 hpf, gDNA extracted from TDCPP-treated embryos was incompletely digested by all methylation-sensitive restriction endonucleases tested, and banding patterns were similar to those of vehicle-treated controls (Figure 6).
In recent years, cytogenetic studies in bivalves have expanded as a result of the introduction of new molecular cytogenetic techniques such as in situ restriction endonuclease (RE) banding and fluorescence in situ hybridization (FISH).
coli protein expression vector using polymerase chain reaction (PCR), restriction endonuclease digestion, and DNA ligation.
Fragile X is typically diagnosed by analysis of the number of CGG repeats and their methylation status using restriction endonuclease digestion, polymerase chain reaction, and Southern blot analysis.
A primitive form of immune system, R-M systems consist of a restriction endonuclease and a matching modification enzyme, which recognises and modifies (generally by methylation) the DNA sequence recognised by the restriction endonuclease.
Use of plasmid profiles and restriction endonuclease digest in enviroronmental studies of Listeria spp.
A mutation site in the ITS1 region among BF cirrhosae and other species of BF has been found and can be recognized by the restriction endonuclease SmaI.

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