molecular identification, randomly amplified polymorphic DNA, restriction fragment
DNA Profiles can be forensically analyzed by restriction fragment
length polymorphism (RFLP) method, polymerase chain reaction (PCR) method, short tandem repeat (STR), mtDNA Profiling and DNA Chip technology.
Molecular identification was conducted at Seoul National University Bundang Hospital, where PCR restriction fragment
length polymorphism and multiplex real-time PCR and melting curve analyses were performed as described (5,6).
Variation in restriction fragment
length polymorphism could be seen.
The amplified restriction fragment
length polymorphism (AFLP) technique is a relatively new genotypic method that has not yet been widely used in parasitology (13).
For [lambda] DNA sequences, we counted the number of reads mapped to each restriction site and ignored sites with restriction fragment
lengths of <30 bp (because 25 bp was used for alignment).
Using terminal restriction fragment
length polymorphism (TRFLP) analysis of PCR-amplified 16S-rDNA sequences, we characterized eubacterial community structure in soil collected on the Bloomington campus of Indiana University beneath native Canadian wildginger (Asarum canadense L.
Morphological analysis included investigating the ratio of three morphometric variables, while the genetic data relied on restriction fragment
length polymorphism analysis through the digestion of the mitochondrial sequences of the COI gene.
Initial techniques that harnessed DNA for species identification in the food industry have included random amplified polymorphic DNA (RAPD) and restriction fragment
length polymorphism (RFLP) assays.
A 100 base-pair (bp) ladder provided molecular weight markers to estimate restriction fragment
Techniques mastered during the program include agarose, polyacrylamide and protein gel electrophoresis, DNA sequencing, gene mapping, restriction enzyme cleavage of DNA, recombinant gene technology, cloning, polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (rtPCR), Southern and Western blotting, analysis of restriction fragment
polymorhisms (RFLP), analysis of variable number tandem repeats (VNTR's), DNA fingerprinting for paternity and forensics analysis, protein analysis and bioprocessing.
15) These lineages, or haplogroups, are defined by specific restriction fragment
length polymorphisms and base pair deletions.