restriction fragment


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restriction fragment

n
(Genetics) genetics a fragment of a DNA molecule cleaved by a restriction enzyme. See also RFLP
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.restriction fragment - the fragment of DNA that is produced by cleaving DNA with a restriction enzyme
fragment - a piece broken off or cut off of something else; "a fragment of rock"
Translations
fragment de restriction
References in periodicals archive ?
molecular identification, randomly amplified polymorphic DNA, restriction fragment length polymorphism
DNA Profiles can be forensically analyzed by restriction fragment length polymorphism (RFLP) method, polymerase chain reaction (PCR) method, short tandem repeat (STR), mtDNA Profiling and DNA Chip technology.
Molecular identification was conducted at Seoul National University Bundang Hospital, where PCR restriction fragment length polymorphism and multiplex real-time PCR and melting curve analyses were performed as described (5,6).
Variation in restriction fragment length polymorphism could be seen.
The amplified restriction fragment length polymorphism (AFLP) technique is a relatively new genotypic method that has not yet been widely used in parasitology (13).
For [lambda] DNA sequences, we counted the number of reads mapped to each restriction site and ignored sites with restriction fragment lengths of <30 bp (because 25 bp was used for alignment).
Using terminal restriction fragment length polymorphism (TRFLP) analysis of PCR-amplified 16S-rDNA sequences, we characterized eubacterial community structure in soil collected on the Bloomington campus of Indiana University beneath native Canadian wildginger (Asarum canadense L.
Morphological analysis included investigating the ratio of three morphometric variables, while the genetic data relied on restriction fragment length polymorphism analysis through the digestion of the mitochondrial sequences of the COI gene.
Initial techniques that harnessed DNA for species identification in the food industry have included random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) assays.
Techniques mastered during the program include agarose, polyacrylamide and protein gel electrophoresis, DNA sequencing, gene mapping, restriction enzyme cleavage of DNA, recombinant gene technology, cloning, polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (rtPCR), Southern and Western blotting, analysis of restriction fragment polymorhisms (RFLP), analysis of variable number tandem repeats (VNTR's), DNA fingerprinting for paternity and forensics analysis, protein analysis and bioprocessing.
15) These lineages, or haplogroups, are defined by specific restriction fragment length polymorphisms and base pair deletions.

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