reverse transcription


Also found in: Thesaurus, Medical, Acronyms, Encyclopedia, Wikipedia.

reverse transcription

n.
The process by which DNA is synthesized from an RNA template by means of the enzyme reverse transcriptase.
Mentioned in ?
References in periodicals archive ?
Differential display reverse transcription PCR (DDRT-PCR), introduced firstly by Liang and Pardee (Liang and Pardee, 1992), can identify the total number of genes subject to up- or down-regulation in a particular setting and has been widely used to screen for genes that are differentially expressed (Breyne and Zabeau, 2001; Ahmed et al.
RNA from 4 week-old mouse testis was used as a positive control (+), and RNA from 4 week-old ovary without reverse transcription (RT) was used as a negative control (-).
A kit for genotyping of HCV RNA in serum and plasma based on reverse transcription and PCR analysis Real-time detection of genotypes 1, 2, 3, 4, 5 and 6 and subtypes 1a, 1b, 24 reactions.
Characteristics of 24 patients with positive and negative results for infection with Zika virus, Brazil, 2015 Reverse transcription Mean (SD) No.
For these reasons, it is essential that the reporting of results involving cDNA syntheses is as transparent and complete as possible and includes the amount of RNA reverse-transcribed, priming strategy, enzyme type, volume, temperature, and duration of the reverse transcription step.
Drugs that blocked viral entry or that blocked the start of reverse transcription stopped the killing.
The test employs real-time reverse transcription (RT) polymerase chain reaction (PCR) to detect the RNA of the 2009 H1N1 flu virus (2009 H1N1) in a patient's nasal or nasopharyngeal specimens.
reverse transcription, transcriptional latency, AIDS.
The goal of this study was to further elucidate the regulatory mechanisms of Dnmt activity by (i) measuring messenger RNA (mRNA) transcription with Reverse Transcription Polymerase Chain Reaction (RT-PCR), and (ii) Dnmt protein synthesis with immunobloting.
Reverse transcription and polymerase chain reactions were performed to determine if bphA and bphE are on the same transcript, or different transcripts.

Full browser ?