1) converts ribonucleoside
diphosphates into deoxyribonucleoside diphosphates being essential for DNA replication, which is why the enzyme is considered an excellent target for anticancer therapy.
3% trichloroacetic acid in dichloromethane) followed by coupling of a ribonucleoside
phosphoramidite in the presence of an activating reagent (i.
Hydroxyurea, which is an inhibitor of ribonucleoside
reductase and is used as a systemic antitumor agent, is frequently used for the treatment of myeloproliferative and hematologic diseases.
Among their perspectives are inhibiting HIV entry, helicase-primase inhibitors as a new approach to combating herpes simplex virus and varicella zoster virus, alkoxyalkyl ester pro-drugs of antiviral nucleoside phosphate and phosphonates, maribavir as a novel benzimidazole ribonucleoside
for preventing and treating cytomegalovirus diseases, lethal mutagenesis as an unconventional approach to combating HIV, silencing viruses with antiviral RNAi, and neuraminidase inhibitors as anti-influenza agents.
NS5B nucleoside inhibitors (NIs) or analogs (NM-107/NM-283, PSI 6130/R7128, IDX184, MK-0608) compete with cellular ribonucleoside
triphospahtes act as functional chain terminators (114).
Efficiency of digestion was confirmed by HPLC against ribonucleoside
The primary follicle culture medium was consisted of ribonucleoside
and deoxyribonucleoside-free [alpha]-MEM-glutamax medium supplemented with 1% (v/v) heat-inactivated FBS, 1% (v/v) ITS liquid medium, 100 mIU/ml recombinant human FSH, and 10 mIU/ml LH, to which 0, 5, 10, 50, or 100 [micro]M bME were added.
The method uses an oligonucleotide having a sequence essentially identical to a guide sequence found in the 5 flanking exon and terminates with a 3 ribonucleoside
The DNA polymerase recognizes the base sequence to which it must bind and align the ribonucleoside
triphosphates with the complementary bases.
As a ribonucleoside
, azacytidine would mainly incoporate into RNA.
The 20-[micro]L transcription mixture included 2 [micro]L each of the ribonucleoside
triphosphates (containing 10 mmol/L each of ATP, CTP, TTP, and GTP), transcription buffer, and T7 RNA polymerase (20 U/[micro]L; Roche), along with 1 [micro]L of RNase inhibitor (20 units/[micro]L; Applied Biosystems).
The broad spectrum antiviral ribonucleoside
ribavirin is an RNA virus mutagen.