NeoSeek's Salmonella serotyping
service provides a much quicker, definitive alternative to the conventional biochemical identification of Salmonella serotypes from presumptively positive Salmonella isolates.
Assay costs were from $10 to $12 per sample using ISR, compared with $35 to $185 for KW serotyping
This phenomenon has contributed to the huge diversity of Salmonella serotypes and dictates that the serotyping
system sometimes poorly reflects true phylogeny.
Invasive site isolates are voluntarily and routinely submitted to the BCCDC Public Health and Reference Microbiology Laboratory by front-line hospital and community laboratories for serotyping
and forwarded to a national reference laboratory for confirmation (National Centre for Streptococcus 2002-April 2010, and National Microbiology Laboratory April to December 2010).
of pneumococcal isolates causing meningitis in children <5 years identified 78% (281/360) as serotypes in PCV-7.
Genetic analysis methods like ribotyping, pulse field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have shown additional discriminatory power over serotyping
Isolation and serotyping
was conducted as recommended by the World Health Organization (WHO) with three cell lines: 1) L20B cells, a mouse L-cell line genetically engineered to express the human poliovirus receptor; 2) RD cells, derived from a human rhabdomyosarcoma; and 3) HEp-2 Cincinnati cells, derived from a human epidermoid carcinoma of the larynx (WHO, 2000).
Lack of utility of serotyping
multiple colonies for detection of simultaneous nasopharyngeal carriage of different pneumococcal serotypes.
Their initial approach was to develop a DNA microarray chip for identifying Listeria species and for the partial serotyping
and genotyping of L.
11) Such organisms (eg, Staphylococcus aureus, Escherichia coli, Enterococcus species) are not routinely subspeciated, as are the Salmonella, by serotyping
On December 18, 1998, the CDC received the results from serotyping
which confirmed, serotype 4b.
Although genetic heterogeneity and differences in reservoirs exist within serotypes (22,37), our results demonstrate that serotyping
provides helpful discrimination among certain serotypefood commodity pairs.