Biochemical characterization of strain B14 Characteristic Strain B14 Colony pigmentation Creamy Motility Utilization of: + Lactose + Xylose + Maltose + Fructose + Dextrose + Galactose + Raffinose + Trihalose + Melobiose + Sucrose + L-arabinose + Mannose - Inulin + Sodium gluconate + Glycerol + Salicin - Dulcitol - Inositol - Sorbitol - Mannitol + Adonitol - Arabitol - Erythritol - a-methyl-D-glucoside - Rhamnose - Cellobiose + Melezitose + a-methyl-D-mannoside - Xylitol - D-arabinose + Citrate - Malonate - Sorbose
- ONPG hydrolysis + Esculin hydrolysis + Table 2.
Isolates were replica plated in microtiter plates containing 180 [micro]l of phenol red broth base (BBL) supplemented with 1% sugar solutions (arabinose, mannitol, methyla-[alpha]-glucopyranoside (MGP), ribose, sorbose
, or sorbitol) and incubated at 37[degrees]C for 24 h.
Further identification to species level were based on carbohydrate fermentation using 1% solution of following sugars: glucose, lactose, mannitol, sucrose, arabinose, sorbose
, sorbitol, raffinose, ribose, trehalose, xylose, melibiose, glycerol; by pigment production, motility test, pyruvate utilization in 1% pyruvate broth, acidification of methyl-alpha-D-glucopyranoside, Voges-Proskeuer test, arginine decarboxylation, hippurate hydrolysis, reduction of potassium tellurite and tetrazolium chloride.
The biochemical tests used to identify enterococci species was Purple Broth Base (Difco, Detroit, USA) supplemented with arabinos, MGP (Metil-[alpha]-Dglycopiranoside), arginine, mannitol, sorbitol, sorbose