I discovered that the repeat region is translated in all three reading frames into aggregating dipeptide-repeat (DPR) proteins despite its intronic localization and lack of an ATG start codon
Tusavims has a potential upstream start codon
MSS in a weaker Kozak consensus sequence than MAQ (Figure, panel A), which we selected as the start codon
All these polymorphisms are numbered relative to the adenine of the translation start codon
ORF 2 was originally reported to commence at nucleotide position 4,390 at a canonical AUG start codon
Textbooks of my college era discussed genes in terms of proposed models of cistrons, recons, and mutons; were missing the start codon
in charts of the genetic code; and listed "nonsense" for what we know as stop codons (Keeton, 1967).
The first exon is untranslated, and the start codon
(AUG) is located at the beginning of exon 2 (1).
This polymorphism, resulting from a T[right arrow]C transition, introduces codon 4 as the start codon
resulting in a shorter isoform of the VDR.
The GSTP1 313A[right arrow]G (rs1695), NQO1 609C[right arrow]T (rs1800566), MBL2 codon 54A[right arrow]G (rs1800450), ABCB1 3435C[right arrow]T (rs1045642), MTHFR 677C[right arrow]T (rs1801133), and VDR start codon
FokI polymorphism (rs2228570) were genotyped by DNAPrint Genomics (Sarasota, FL) in a multiplex PCR followed by single base extension.
Patients were genotyped for VDR gene polymorphisms at the translation start codon
and at the 3'-end region.
Next, each ORF segment was aligned with the start codon
beginning at the zero location (ORFs indicated as being in the opposite direction were computed as reverse compliments and aligned).
Primers (200ug/ml) were designed flanking the c1 gene and contained a ribosome binding site upstream of the c1 gene start codon
The AUG mRNA codon codes for methionine and also acts as the start codon
for all proteins.