supravital


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su·pra·vi·tal

 (so͞o′prə-vīt′l)
adj.
Of, relating to, or capable of staining living cells after their removal from a living or recently dead organism: a supravital dye.

supravital

(ˌsuːprəˈvaɪtəl)
adj
(Pathology) (of a stain) relating to living tissue outside of the body

su•pra•vi•tal

(ˌsu prəˈvaɪt l)

adj.
pertaining to or involving a staining method for a preparation of living cells.
[1915–20]
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References in periodicals archive ?
Briefly described as a small drop of frozen-thawed semen was placed on a pre-warmed slide and mixed with a relatively larger drop of the supravital stain [1% (w/v) eosin B, 5% (w/v) nigrosin dissolved in 3% tri-sodium citrate dehydrate solution] to prepare a thin and uniform smear.
When this is requested, an aliquot is mixed with a supravital stain before the slide is produced, stained, and imaged.
6] CSGMT (The Collaborative Study Group for the Micronucleus Test), "Micronucleus test with mouse peripheral blood erythrocytes by acridine orange supravital staining: The summary report of the 5th collaborative study by CSGMT/JEMS.
Some, such as haemoglobin H inclusions and Heinz bodies can only be appreciated with supravital staining [14].
A cross sectional study was conducted to compare the FNAC smears adequacy by toluidine blue, a supravital stain (TBS) and Papanicolaou stain (PAPS).
The reticulocyte count was determined from blood smears after supravital staining with methylene blue and reported as a percentage of circulating RBCs.
The percentage of normal nonapoptotic cells and apoptotic cells was measured by double supravital staining with Annexin-V and propidium iodide (PI) following manufacturer's instruction, using an annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (KeyGen, Nanjing, China).
Esta situacion permite postular que soluciones de osmolaridad muy baja, causarian ademas de la respuesta fisiologica de equilibrio osmotico inicial, una mayor cantidad de dano fisico en los espermatozoides en el tiempo; esto considerando que la tincion supravital mide la ruptura de la membrana plasmatica en el espermatozoide muerto--integridad fisica-, mientras que HOST evalua la integridad funcional de la misma--integridad bioquimica(Schrader et al, 1986).
FBC and reticulocyte count (automated Sysmex XE 5000, analyser; Sysmex Incorporated, Japan), blood smears for microscopy (automated Sysmex 1000i slide maker and stainer; Sysmex Incorporated, Japan), and reticulocyte preparation for microscopy (2-hour incubation with 1% Brilliant Cresyl Blue supravital stain; National Health Laboratory Service, South Africa).
Results: Of the 114 aspirates included in our study the diagnostic accuracy by using papanicolaou was 78%, while it was upto 100% with supravital toluidine blue stained smears.
The haematoxylin and eosin staining in contrast to the usual supravital staining facilitated a permanent preparation and easy comparison of smear with the paraffin sections for accurate interpretation.