Briefly described as a small drop of frozen-thawed semen was placed on a pre-warmed slide and mixed with a relatively larger drop of the supravital
stain [1% (w/v) eosin B, 5% (w/v) nigrosin dissolved in 3% tri-sodium citrate dehydrate solution] to prepare a thin and uniform smear.
When this is requested, an aliquot is mixed with a supravital
stain before the slide is produced, stained, and imaged.
6] CSGMT (The Collaborative Study Group for the Micronucleus Test), "Micronucleus test with mouse peripheral blood erythrocytes by acridine orange supravital
staining: The summary report of the 5th collaborative study by CSGMT/JEMS.
Some, such as haemoglobin H inclusions and Heinz bodies can only be appreciated with supravital
A cross sectional study was conducted to compare the FNAC smears adequacy by toluidine blue, a supravital
stain (TBS) and Papanicolaou stain (PAPS).
The reticulocyte count was determined from blood smears after supravital
staining with methylene blue and reported as a percentage of circulating RBCs.
The percentage of normal nonapoptotic cells and apoptotic cells was measured by double supravital
staining with Annexin-V and propidium iodide (PI) following manufacturer's instruction, using an annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (KeyGen, Nanjing, China).
Se evaluo mediante la prueba supravital
con eosina-nigrosina (VE) (Barth y Oko 1989).
Esta situacion permite postular que soluciones de osmolaridad muy baja, causarian ademas de la respuesta fisiologica de equilibrio osmotico inicial, una mayor cantidad de dano fisico en los espermatozoides en el tiempo; esto considerando que la tincion supravital
mide la ruptura de la membrana plasmatica en el espermatozoide muerto--integridad fisica-, mientras que HOST evalua la integridad funcional de la misma--integridad bioquimica(Schrader et al, 1986).
FBC and reticulocyte count (automated Sysmex XE 5000, analyser; Sysmex Incorporated, Japan), blood smears for microscopy (automated Sysmex 1000i slide maker and stainer; Sysmex Incorporated, Japan), and reticulocyte preparation for microscopy (2-hour incubation with 1% Brilliant Cresyl Blue supravital
stain; National Health Laboratory Service, South Africa).
Results: Of the 114 aspirates included in our study the diagnostic accuracy by using papanicolaou was 78%, while it was upto 100% with supravital
toluidine blue stained smears.
The haematoxylin and eosin staining in contrast to the usual supravital
staining facilitated a permanent preparation and easy comparison of smear with the paraffin sections for accurate interpretation.