Manual evaluation of urine sediment may be further enhanced by using supravital
stains such as crystal violet/safranin O stain and phase miscroscopy.
2,3) The initial description of retPLTs dates from 1969, when Ingram and Coopersmith (4) observed coarsely punctuated reticulum in canine platelets after supravital
staining with new methylene blue.
The reference staining method for the manual reticulocyte count uses a dye such as new methylene blue and supravital
staining differentiates between live and dead sperm and is assessed when sperm motility is <50%.
A 50-[micro]L drop of frozen-thawed semen was placed on a pre-warmed slide and mixed with 50 [micro]l drop of the supravital
stain [1% (w/v) eosin B, 5% (w/v) nigrosin in 3% tri-sodium citrate dehydrate solution] to prepare a thin smear .
Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry and Supravital
Dyes); Approved Guideline- Second Edition H44-A2.
exposure to propidium iodide identities apoptotic cells in the absence of nucleosomal DNA fragmentation.