These results confirm that a topical application of our toluidine
blue, rather than the traditional systemic administration of a photosensitizer and its attendant side effects, may provide an effective alternative to current PDT modalities.
Reds (DPP, Naphthols, Anthraquinone, Toluidine
, Anthanthrone, Bon Maroon Quinacridone) Yellow and Oranges (Bismuth Vanadale, Diarylide, Monoflag, Benzimidazolone, Hybrids, Isoindolinone, Dianisidine)
Serum treponema pallidum particle agglutination test positive, other syphilis relative tests such as serum toluidine
red unheated serum test, rapid plasma reagin (RPR), Venereal Disease Research Laboratory (VDRL), and CSF RPR, VDRL tests were all negative.
The roots, hypocotyls, epicotyls, cotyledons and the eophylls were fixed in FAA 50 (formaldehyde, glacial acetic acid and ethyl alcohol) (JOHANSEN, 1940) and Glutaraldehyde (KARNOVSKY, 1965), embedded in historesin (GERRITS, 1991), cross-sectioned on a rotation microtome and stained with toluidine
blue (O'BRIEN et al.
Dai published an interesting observation : the diffusion of a toluidine
blue colloid solution in water measured over a 3-year time span showed anisotropic patterns, i.
figure 10) The resected specimen of the mass was subjected to thorough histopathological examination by special stains like periodic acid-Schiff (PAS), silver impregnation for reticulin, Bodian's stain for nerve fibres and toluidine
blue to rule out other differential diagnoses & to reach to a final conclusion.
Eight micrometer sections were made using a Reichert-Jung Leica model 2040 Autocut, and the following techniques were used: Toluidine
Blue (routine), Periodic Acid of Schiff (PAS, carbohydrates), Bromophenol Blue (proteins) (Pearse, 1985) and Nile Blue (lipids) (Hawes and Satiat-Jeunemaitre, 2001).
The sections were stained with toluidine
blue/basic fuchsin stain (1% toluidine
blue O [C.
Other screening and diagnostic methods include the use of chemiluminescent light, mucosal staining with blue dye called toluidine
blue, brush biopsy, incisional biopsy and punch tissue biopsy.
5% aqueous solution of Toluidine
Blue to enhance the contrast of the wood tissue.
Six sections were obtained from each block, stained with toluidine
Briefly, 8-[micro]m-thick sections were cut with a microtome and stained with hematoxylineosin and toluidine