transfect


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trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.

transfect

(trænsˈfɛkt)
vb
(Genetics) (tr) to bring about transfection in
[from trans- + (in)fect]

transfect


Past participle: transfected
Gerund: transfecting

Imperative
transfect
transfect
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Preterite
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Present Continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present Perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Past Continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past Perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future Perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future Continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Present Perfect Continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Future Perfect Continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
Past Perfect Continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Past Conditional
I would have transfected
you would have transfected
he/she/it would have transfected
we would have transfected
you would have transfected
they would have transfected
References in periodicals archive ?
The research team at ImmunoCellular successfully packaged a T cell receptor (TCR) DNA sequence into a lentiviral vector, which was then used to transfect human hematopoietic stem cells.
Especially designed for primary cells and very difficult to transfect cell types.
Lipofectamine MessengerMAX is up to five times more efficient than DNA reagents, allowing researchers to easily transfect biologically relevant cell models without electroporation or viruses.
Using lentiviral particles also allows effective direct visualization of Nuclei, Nuclear Membrane, Mitotic Chromosomes and Interphase Chromatin, Endosome, Endoplasmic Reticulum, Microtubule, Mitochondria, Golgi, Lysosome, Plasma Membrane, Cytoplasm and Peroxisome in hard to transfect mammalian cells, stem cells and primary cells.
It is also not easy to transfect oviduct cell due to unavailability of cell line.
By using the MaxCyte STX scalable transfection system, CCS Cell Culture Service is able to transfect batches of up to 10 billion cells in [less than]30 min.
A key step in bringing this vector to the clinic was to demonstrate that it is both safe and feasible to transfect the vector into human stem cells and for the RNA molecules to persist in the immune cells of the patient.
The Helios is designed to transfect in vivo cells and has a pressure range of 100-600 psi.
The first experiment devoted to transfect the male gamete was done in the rabbit by Brackett et al.
The ability to transiently transfect 2E11 mammalian cells with RNA or DNA using a single 30 minute run is unique in the industry and aligns perfectly with Paragon's expertise in protein, virus, and vaccine production.
5 [micro]g/well) and 60 nmol of pre-miR negative control or pre-miR has-miR-206 (Ambion, USA) were premixed, and then the mixture was used to transfect 293 cells.
The hek 293 cells are very easy to work with, which means they can be straightforwardly grow in culture and to transfect, and are very popular as hosts for gene expression.