transfect

(redirected from transfectants)

trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.

transfect

(trænsˈfɛkt)
vb
(Genetics) (tr) to bring about transfection in
[from trans- + (in)fect]

transfect


Past participle: transfected
Gerund: transfecting

Imperative
transfect
transfect
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Preterite
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Present Continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present Perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Past Continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past Perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future Perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future Continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Present Perfect Continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Future Perfect Continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
Past Perfect Continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Past Conditional
I would have transfected
you would have transfected
he/she/it would have transfected
we would have transfected
you would have transfected
they would have transfected
References in periodicals archive ?
Overexpression of survivin in these stable transfectants was verified by immunoblotting.
The transfectants were transferred and maintained in DMEM supplemented with 10% FBS and 400 mg/ml G418.
We transfected Chinese hamster ovary (CHO) cells (American Type Culture Collection) with the human EL-c-myc/pHBAP-3-neo plasmid (10) and selected stable transfectants with 500 [micro]g/mL G418 (Invitrogen).
The stable transfectants were established and cultured in antibiotic-free DMEM for at least two passages before performing experiments.
Generally transfectants do not express sufficient levels of fluorescence for spectrofluorometric measurement on micro plate.
The transfectants were still expressing the marker gene, more than 30 days post transfection, when the study was terminated.
The broad dynamic range of the Guava Express assay provides the precise discrimination necessary for studying cell receptor up/down-regulation, screening transfectants, evaluating whole blood lymphocyte populations, and many other applications.
ELISA assay showed that the transfectants secreted hTPO protein in media at the level of 774 pg/ml (pBCTPOKI-6) and 1,867 pg/ml (pBCTPOKI-10), respectively (Figure 1C).
After stable transfection of HEK-293 cells with the a, [beta], and [gamma] subunits of the human Fc[epsilonRI, we identified transfectants expressing the functional receptor by use of FRCS using an [alpha] chain-specific antibody (Fig.
Mouse epidermal JB6 Cl41 cells and their stable transfectants were cultured in Eagle's minimal essential medium (MEM; Calbiochem, San Diego, CA) supplemented with 5% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 2 mM L-glutamine (Life Technologies, Inc.
In addition to the parental cell line preparation, which produced a moderate amount of BCAR1, the study included the transfectant 4A12, which overproduced BCAR1 (1), and transfectants that overproduced FLAG-tagged (Stratagene Europe) BCAR1 (FBCAR1), HEF1, and a FLAG-tagged hybrid molecule consisting of the first 63 amino acid residues of BCAR1 and residues 64-834 of HEF1 (F-Hybrid).
This assay uses MCF7-AR1 cells, which are stable transfectants of MCF7 cells expressing the wild-type human androgen receptor (Szelei et al.