Cells were plated onto 10-cm dishes and transfected
for 24 hr before treatments using X-tremeGene 9 reagent (Roche Diagnostics).
Although there are suggestive clinical data, preclinical data based on GLP-1 receptor staining are unreliable, because the polyclonal antibodies generally used don't consistently stain GLP-1 transfected
The fundamental principles of the nuclear transfer method were experimentally verified by the production of a human factor IX transgenic sheep using transfected
fetal fibroblasts (Schnieke et al.
Cell transfection and generation of cells stably transfected
with pGenesil-ERa small hairpin RNA (shRNA), pGenesil-ER[beta] shRNA and pGenesil-scrambled shRNA vectors
We used HEK293 cells transfected
with Epac FRET probe to optimize our hyperspectral imaging and analysis approaches and to design automated cell analysis techniques to determine the subcellular localization of FRET signals.
Results: Down-regulation of miR-92a in APL cell line (HL-60) by LNA antagomir extensively decreased cell viability in APL Cell viability gradually decreased over time as the viability of LNA-anti-miR transfected
cells was less than 50% of untreated cells at 72 h post-transfection.
Selected lines will be transfected
with Lac Z reporter gene and microtransplanted into various brain regions.
Moreover, PJ did not enhance promoter activity in the eNOS gene (COS-7 cells transfected
Polarized cells, either differentiated (for example, Caco-2 cells) or transfected
with the transporters of interest (such as MDCKII, HEK293, LLCPK cells) are often used to characterize efflux drug transporter interactions.
Gastroenterology- Gliadin GAF(3X), EmA, CIBD-IFA Transfected
cell technology & more.
We separated proteins from the conditioned media of HEK 293 cells transfected
with the plasmid expressing proBNP-T71A by size-exclusion chromatography using a Superdex 75 10/300 GL column (GE Healthcare) as described (10).
The study recruited five patients with AIDS-related lymphoma, four of whose blood stem cells were harvested, transfected
with the triple vector RNA therapeutic and then reinfused back into the patients.