To study the functional role of DcR3 in liver cancer, we stably knocked down DcR3 expression in HepG2 cells by transfecting
the cells with DcR3 shRNA using lentivirus.
10 This study aims at detecting the effects of HCV NS2 on oncogene expression including intracellular Bcl-2 and Bax by transfecting
HepG2 cell with NS2 and C gene eukaryotic expression vectors so as to explore the role of NS2 in the occurrence of cirrhosis and cancer induced by HCV infection.
Expressions of Bc1-2, MMP2 and 1EGF in ASCs after transfecting
with IP-10 plasmid
50ul of pL-OV1345tPAGFP and pL-OV2964tPAGFP viruses were used for transfecting
equal number of immortalised oviduct epithelial cells, respectively.
For the positive control generated by transfecting
with pCMV-VifFLAG alone, 28 | g of total protein was added due to a smaller yield in this sample.
HIF-1[alpha]]: RNA interference targeting HIF-1[alpha] was performed by transfecting
This non-viral method is also ideal for transfecting
a wide range of hard-to-transfect cells.
Topics include AMPA receptor trafficking, long-term plasticity at inhibitory synapses, proteomics of the PSD, phosphorylation of site-specific antibodies, protein palmitoylation by the DHHC protein family, antibody labeling and biotinylation approaches to study neurotransmitter receptors, visualization of AMPAR trafficking and surface expression, neurotransmitter dynamics, receptors dynamics and functional tagging, RNAi and applications in neurobiology, transfecting
and transducing neurons, expression of recombinant proteins in rat brains using Sindbus virus, Lentivirus-based genetic manipulation, AMPA receptor phosphorylation in synaptic plasticity, and genomic and post-genomic tools for studying synapse biology.
The second obstacle was the difficulty of transfecting
the cell line with plasmids and growing enough virus to be of use as seed stock.
The cDNA and vector were rescued by transfecting
the mouse cells with packaging vector and vesicular stomatitis virus G (VSV G).
Whether our attention, and our action, is addressed to labor systems, sexual configurations, circuits of disease and well-being, banking wizardry, trajectories of food and the means to get it, political organizing, religious visions, virtual realities and time-space compressions symbolized by the internet, racial formations, reality or the Reality Engine[TM], serious business or world-shaping play, cyborg figures have a way of transfecting
, infecting, everything.
Because the new TransCytos transfection technology is gentle, highly effective, and does not physically damage cells, it is potentially capable of transfecting
all cell types," says Dr.