transfect

(redirected from transfecting)

trans·fec·tion

 (trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.


trans·fect′ v.

transfect

(trænsˈfɛkt)
vb
(Genetics) (tr) to bring about transfection in
[from trans- + (in)fect]

transfect


Past participle: transfected
Gerund: transfecting

Imperative
transfect
transfect
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Preterite
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Present Continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present Perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Past Continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past Perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future Perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future Continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Present Perfect Continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Future Perfect Continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
Past Perfect Continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Past Conditional
I would have transfected
you would have transfected
he/she/it would have transfected
we would have transfected
you would have transfected
they would have transfected
References in periodicals archive ?
To study the functional role of DcR3 in liver cancer, we stably knocked down DcR3 expression in HepG2 cells by transfecting the cells with DcR3 shRNA using lentivirus.
10 This study aims at detecting the effects of HCV NS2 on oncogene expression including intracellular Bcl-2 and Bax by transfecting HepG2 cell with NS2 and C gene eukaryotic expression vectors so as to explore the role of NS2 in the occurrence of cirrhosis and cancer induced by HCV infection.
Expressions of Bc1-2, MMP2 and 1EGF in ASCs after transfecting with IP-10 plasmid
50ul of pL-OV1345tPAGFP and pL-OV2964tPAGFP viruses were used for transfecting equal number of immortalised oviduct epithelial cells, respectively.
For the positive control generated by transfecting with pCMV-VifFLAG alone, 28 | g of total protein was added due to a smaller yield in this sample.
HIF-1[alpha]]: RNA interference targeting HIF-1[alpha] was performed by transfecting 50nM [siRNA.
This non-viral method is also ideal for transfecting a wide range of hard-to-transfect cells.
Topics include AMPA receptor trafficking, long-term plasticity at inhibitory synapses, proteomics of the PSD, phosphorylation of site-specific antibodies, protein palmitoylation by the DHHC protein family, antibody labeling and biotinylation approaches to study neurotransmitter receptors, visualization of AMPAR trafficking and surface expression, neurotransmitter dynamics, receptors dynamics and functional tagging, RNAi and applications in neurobiology, transfecting and transducing neurons, expression of recombinant proteins in rat brains using Sindbus virus, Lentivirus-based genetic manipulation, AMPA receptor phosphorylation in synaptic plasticity, and genomic and post-genomic tools for studying synapse biology.
The second obstacle was the difficulty of transfecting the cell line with plasmids and growing enough virus to be of use as seed stock.
The cDNA and vector were rescued by transfecting the mouse cells with packaging vector and vesicular stomatitis virus G (VSV G).
Whether our attention, and our action, is addressed to labor systems, sexual configurations, circuits of disease and well-being, banking wizardry, trajectories of food and the means to get it, political organizing, religious visions, virtual realities and time-space compressions symbolized by the internet, racial formations, reality or the Reality Engine[TM], serious business or world-shaping play, cyborg figures have a way of transfecting, infecting, everything.
Because the new TransCytos transfection technology is gentle, highly effective, and does not physically damage cells, it is potentially capable of transfecting all cell types," says Dr.