trypan blue


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trypan blue

(ˈtrɪpən; ˈtrɪpæn; trɪˈpæn)
n
(Biology) a dye obtained from tolidine that is absorbed by the macrophages of the reticuloendothelial system and is therefore used for staining cells in biological research
[so called because it is trypanocidal: see trypanosome, -cide]
References in periodicals archive ?
sup][3] Other dyes, such as trypan blue [sup][4] and brilliant blue G,[sup][5] were later introduced as alternatives for ILM staining.
The cell-counting and cell-staining procedures were carried out using a Neubauer chamber and trypan blue (Sigma, U.
The number of viable cells was determined by Trypan blue exclusion test on days 8 and 15 (n = 5).
Trypan blue dye use for better contrast during hexis.
In the present study, we compared the results of the methods using crystal violet staining (1% glutaraldehyde or 4% paraformaldehye fixation), LDH and trypan blue assay to reveal the most suitable assay to determine the effect of hyperthermia on viability of human breast cancer (MDA-MB 231) and normal liver (WRL-68) cells.
RPMI-1640 medium, fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Sigma (Germany), dimethylsulfoxide (DMSO) and trypan blue from Merck (Germany), trypsin/EDTA solution from Gibco (U.
At the end of culture, GC viability was evaluated by trypan blue dye exclusion method.
Cd-induced cytotoxicity and cellular damage were both evaluated by trypan blue exclusion, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT), and lactate dehydrogenase leakage (LDH) assays.
Tabela 1--Medias ([+ or -] desvio padrao) da Motilidade Espermatica (%) na hora quatro de descongelacao; do CFDA-PI (%) e do Trypan Blue Giemsa (%) na hora zero (H0) da descongelacao--Motilidade (MOT), Membranas integras (IMP), Espermatozoides Vivo integro (VI); Vivo reagido (VR); Morto integro (MI) e Morto reagido (MR).
Cell number and viability were determined by staining the cells with trypan blue dye (Sigma, Germany) and counted using a hemocytometer viewed under an inverted light microscope.
In addition, viability of isolated neutrophils was tested with Trypan blue solution and percentage of viable cells was counted using a hemocytometer at a 400 fold total magnification.