sup] Other dyes, such as trypan blue
[sup] and brilliant blue G,[sup] were later introduced as alternatives for ILM staining.
The cell-counting and cell-staining procedures were carried out using a Neubauer chamber and trypan blue
The number of viable cells was determined by Trypan blue
exclusion test on days 8 and 15 (n = 5).
dye use for better contrast during hexis.
Total cell number and cell viability were determined after trypan blue
In the present study, we compared the results of the methods using crystal violet staining (1% glutaraldehyde or 4% paraformaldehye fixation), LDH and trypan blue
assay to reveal the most suitable assay to determine the effect of hyperthermia on viability of human breast cancer (MDA-MB 231) and normal liver (WRL-68) cells.
RPMI-1640 medium, fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Sigma (Germany), dimethylsulfoxide (DMSO) and trypan blue
from Merck (Germany), trypsin/EDTA solution from Gibco (U.
At the end of culture, GC viability was evaluated by trypan blue
dye exclusion method.
Cd-induced cytotoxicity and cellular damage were both evaluated by trypan blue
exclusion, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT), and lactate dehydrogenase leakage (LDH) assays.
Tabela 1--Medias ([+ or -] desvio padrao) da Motilidade Espermatica (%) na hora quatro de descongelacao; do CFDA-PI (%) e do Trypan Blue
Giemsa (%) na hora zero (H0) da descongelacao--Motilidade (MOT), Membranas integras (IMP), Espermatozoides Vivo integro (VI); Vivo reagido (VR); Morto integro (MI) e Morto reagido (MR).
Cell number and viability were determined by staining the cells with trypan blue
dye (Sigma, Germany) and counted using a hemocytometer viewed under an inverted light microscope.
In addition, viability of isolated neutrophils was tested with Trypan blue
solution and percentage of viable cells was counted using a hemocytometer at a 400 fold total magnification.