Samples for transmission electron microscope (TEM) analysis were prepared by cryocutting using an ultramicrotome
(Leica, Ultracut UCT) prior to analysis.
The University of Glasgow has a requirement to purchase a state of the art ultramicrotome
for ultrathin sectioning of biological samples with a stereo microscope carrier, anti-vibration table and a touch-sensitive-control unit and a LED-illumination.
High-quality transverse surfaces of the bondline were prepared for nanoindentation with a diamond knife fit in an ultramicrotome
(Jakes et al.
Semithin transverse sections (1 [micro]m) were cut with an automatic ultramicrotome
(Leica Ultracut R, Austria) and stained with filtered 1% toluidine blue.
Sections of 2-6 [micro]m in thickness were cut from the resin blocks using a Sorvall MT-2b ultramicrotome
, mounted on microscope slides, stained with TBO (Ruzin.
Ultra-thin sections were obtained using diamond knives with a Leica EM UC6 ultramicrotome
Blocks with specific areas were then chosen and sectioned with glass knives on a KBV ultramicrotome
The SFTSV-infected Vero cell monolayer was fixed according to described methods (11) and cut on ultramicrotome
(RMC MT-XL) at 65 nm.
Afterwards, the blocks were shaped as trapezes, having 1 mm on each side and then sliced into sections using an ultramicrotome
Thin sections were cut with an ultramicrotome
and diamond knife and stained with lead citrate.
Specimens were thick sectioned for the presence of the archegonia and thin-sectioned with a diamond knife on an Ultracut-E ultramicrotome
Sections 400 nm thick were cut using an ultramicrotome
(Leica, Germany), stained with 0.