The preparation techniques are the conventional and chemical techniques associated: Polishing, Tripod, Pips, Ultramicrotomy
, Fib .
The sample preparation was carried out using an ultramicrotomy
with a Leica Ultracut UCT (Leica Microsystems GmbH, Vienna, Austria).
The pretreated samples were used for ultramicrotomy
and collected on copper grids, and the obtained sections were then stained and observed using a transmission electron microscope (JEM-2000EX, JEDL, Japan).
We collected thin sections (80 nm) by ultramicrotomy
onto copper 300-mesh thin bar grids and contrasted the sections in lead citrate and uranyl acetate.
The specimens obtained were set in moulds and polymerised at 70[degrees]C for 48 hours forming blocks that were used in ultramicrotomy
Leica will manage the new business from its Ultramicrotomy
facility in Vienna, Austria.