The micro colonies were separated from ungerminated
conidia by filtration through a G-1 sintered glass filter of 120-160 um pore size.
The germination temperature was 25 [+ or -] 1[degrees]C under daylight lamps for seven days, and subsequently we counted data from normal and abnormal seedlings, and ungerminated
seeds (Carneiro & Guedes, 1992a).
Then slides were examined under light microscope to count the germinated and ungerminated
spores with a haemocytometer.
The viability of the ungerminated
seeds was evaluated by the tetrazolium test to 0.
Well, that clover will come up, all right--but it likely will happen the next year, when disking brings the hard, ungerminated
seed to the top of the ground.
A review published by Critical Reviews in Food Science and Nutrition agrees, pointing out that studies have failed to find a substantive nutrient gain when feeding birds sprouted rather than ungerminated
seeds remaining after completion of the germination assay were dead, according to tetrazolium testing.
High level of trypsin inhibitor activity (950 x 103 TIU/g seed) in ungerminated
horsegram seeds reduced by 16% in 72 h germinated seeds (Subbulakshmi et al.
It is not uncommon for some alpine species to shed nondormant seed that remain ungerminated
during autumn due to the prevailing low temperatures (Billings & Mooney, 1968).
After this period, the proportions of germinated and ungerminated
conidia were counted under an optical microscope (400 x).
2011); hence it is possible that ungerminated
buried seeds in our study were dormant, because burial is an essential prelude to dormancy in several species (Thompson et al.
Giami SY and LI Barber Utilization of protein concentrates from ungerminated
and germinated fluted pumpkin (Telfairia occidentalis Hook F.