Cytospin preparations were dehydrated (2x1 min 70% ethanol, 2x1 min 96% ethanol, 2x 1 min 100% ethanol, 2x 5 min xylol
, 1x2 min xylol
) and embedded using Entellan (Merck KGaA).
Histological studies: The left ovaries of 3 groups (8 in each) were fixed in 10% buffered formalin solution, dehydrated with ethanol, cleared with xylol
, impregnated, and embedded in paraffin.
After fixation, the tissues were dehydrated using alcohol, made transparent in xylol
, and after preparation of paraffin blocks, the fragments with 6[micro] in diameter were prepared and studied using an optical microscope after staining with hematoxylin-eosin.
After 6 baths of ethanol (from 70-100%) and 1 of xylol
, the tissues were embedded with paraffin.
0 [micro]m thick) were mounted on slides, deparaffinized in xylol
, and rehydrated in a descending ethanol series for immunostaining.
Name Manufacturer Type Processing solvent Armorseal[R] Sherwin Epoxy MEK/xylene/ethanol 1000 HS clear Williams[R] CARC Clear Spectrum Aliphatic Water MIL-DTL-64159 Ty Coalings polyurethane II CPI (LaRC-CPl) NeXolve[R] Polyimide Diglyme [R] Desolhane[R] HS PRC-DeSoto Urethane Methyl ethyl CA8201/F Clear ketone Irogran[R] Huntsman Thermoplastic Tetrahydrofuran PS455-302P polyurethane (IRO) Pol ycrylic[R] Minwax[R] Acrylic/urethane Water Sylgard 184 Dow Silicone Xylol
0 g) was removed from each gonad, dehydrated in ethanol, cleared in xylol
, and embedded in paraffin.
Peripheral, primary care laboratories would inevitably experience logistical problems relating to the continued and reliable provision of reagents, such as alcohol, xylol
, formalin, and saline, as well as regular maintenance of special equipment.
Tongues were fixed in 10% formalin for paraffin section and dehydrated through 70, 80, 2 x 95, and 3 x 100% alcohol solutions, and further processed through 2 x 100% xylol
and 2 x paraffin wax (60-120 min per step) using a Shandon Gitadel 1,000 Automatic Tissue Processor (Shandon, Manchester, UK).