of adult and prepubertal quail testes homogenate revealed only single band at identical position when subjected to LDH enzyme specific stain (Figure 2).
On the zymogram
two bands were detected, one at a high molecular weight and one at a low weight.
The accuracy/precision of gelatinolytic activities were evaluated by zymogram
densitometry with Image ProPlus software (Cybernetics) (4).
The graph above the zymogram
shows the lack of inhibition of proliferation (MTS assay) at this time point.
1984) were followed for the zymogram
interpretation was achieved following recommendations by Utter et.
Consequently, the content of myocardial enzymes in blood serum increase, so that variation of myocardial zymogram
in blood serum constitutes an index to define damage due to myocardial ischemia.
Karyotype and zymogram
differences among three species of the abalones Haliotis planata, H.
Additionally, we have improved the protocols of electrophoresis, traditionary chromosomal preparation and exogenous gene transfection by trying a local apparatus, different buffers, colchicine and hypotonic time, plasmid DNA incubation time and lipofectamine combination, thus make these techniques feasible under our conditions and greatly improve isoenzyme zymogram
, karyotype quality and exogenous gene transfection efficiency.
Electrophoretic testing for collagenases (Novex Zymogram
Gel) and measurement of urine elastase activity produced results within the reference intervals (data not shown).
and RNA blot analyses show that Sod 4 and Sod 4A are expressed in all the tissues of the maize plant.
MMPs in the medium released from CL1-0 cells were assayed using gelatin zymography (8% zymogram
gelatin gels) according to the methods reported by Huang et al.