Purified trypsin showed a single band on both SDS and zymography
(Figure 2a, b) suggesting their high purity and showing only one dominant isoform of trypsin in Dosidicus gigas hepatopancreas.
of tissue matrix metalloproteinases (MMPs)
3 shows the activity of laccase on SDS-PAGE and the laccase enzyme was successfully oxidizing ABTS substrate to produce two dark green bands for enzyme zymography
is a valuable tool for the visualization of proteolytic activity on an electrophoretic gel matrix, by using a given protein substrate .
To detect metalloproteinase activity, gelatin zymography
was performed by the method described by Unemori and Werb .
Fine-structural distribution of MMP-2 and MMP-9 activities in the rat skeletal muscle upon training: a study by high- resolution in situ zymography
Mannello, "Effects of blood collection methods on gelatin zymography
of matrix metalloproteinases," Clinical Chemistry, vol.
Therefore, we performed gelatin zymography
and western blot assays to determine the changes in MMP-9 activity and protein expression.
The enzymatic activity of MMPs was assessed by zymography
and was enhanced in higher-grade OSCC and along the advancing tumor front.
For MMPs activity assay, fluorescence resonance energy transfer-based MMPs activity assay and gelatin zymography
assay were involved in this study.
was performed in order to determine the MMP-9 activity.
As TIMP2 is required for the efficient activation of proMMP2 by MT1-MMP, immunohistochemistry  and in situ zymography
 demonstrated their co-localization in epithelial carcinomatous and adjacent stromal cells.