Sequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA
bacterial identification system.
For DGGE analysis, approximately 200 bp of the fecal total 16S rDNA
gene was amplified using primers: 338f (5'-ACTCCTACGGGAGGCAGC AG-3') and 533r (5'-TTACCGCGGCTGCTGGCAC-3') and with a 40-base GC clamp (CGCCCGCCGCGCGCGGCGGGCGGGGCGGG GGCACGGGGGG) at the 5' terminus of 338f primer.
tularensis strain was confirmed by real-time PCR that targeted Francisella-specific 16S rDNA
sequences (Francisella LightMix kit; TIP MOLBIOL, Berlin, Germany), a type B-specific real-time PCR that targeted the 23S rDNA gene, as well as 23S rDNA sequencing.
PCR amplification of the 16s rDNA
gene fragments was performed using the universal primers 27f (5' -AGAGTTTGATCMTGGCTCAG-3') and 1492R (5'-ACGGCTACCTTGTTACGACTT-3').
Broad-range 16S rDNA
PCR indeed was negative for all samples in the 'no evidence of infection' group indicating false positivity is not common.
Additionally, genetic characterization was done by sequencing of fragments of the gyrB gene (2) and 16S rDNA
Subsequent analysis by broad-range 16S rDNA
PCR (1) gave a strongly positive result, and the amplicon sequence exactly matched 16S rDNA
sequences from 2 L.
2% identity with Vibrio splendidus ctt 31/5 and Vibrio tasmaniensis 007, respectively, by 16S rDNA
The 16S rDNA
sequences of ZZU strains were compared with sequences from type LAB strains held in the DDBJ.
The scientists were able to identify microbial isolates obtained from fluid milk samples at initial, 7, 14 and 17 days post-processing by using the 16S rDNA
Moreover, partial 16S rDNA
gene sequencing revealed 100% identity across the 3 samples tested, and 99.
Nucleic acids isolated from the biofilm material harvested from the sampling devices will be amplified for the 16S rDNA