Gram-positive, catalase-negative bacilli generating about 200 bp amplicon by PCR with Lactobacillus genus specific primers were further characterized by employing species specific primers followed by sequencing of 16S rDNA
Amplification of 16S rDNA
gene-V3 region, was performed by using the following primers: 16s rDNA
V3, Forward, 5'-CCTACGGGAGGCAGCAG-3' and 16s rDNA
V3, Reverse, 5'-ATTACCGCGGCTGCTGG-3'.
PCR primers F-968-GC (5'-CGCCCGGGG CGCGCCCCGGGCGGGGCGGGGGCACGGG GGGAACGCGAAGAACCTTAC-3') and R-1401 (5'-CGGTGTGTACAAGACCC-3') were combined to amplify the sequence of eubacterial 16S rDNA
from nucleotide 968 to nucleotide 1401.
Moorpark, CA) has patented ammonia-oxidizing bacteria as well as isolated nucleotide sequences representative of 16S rDNA
of these ammonia-oxidizing bacteria.
Conservation within the 16S rDNA
is such that some sequences are shared by all bacteria, some by species of the same genus, and some regions are species specific.
Overall, 60 of the 78 cases of empyema (77%) were microbiologically confirmed either by culture or by 16S rDNA
PCR, and 40 (51%) were found to have pneumococcal origins.
diversities of 16S rDNA
in Palaemonetes pugio populations.
All resulting 16S rDNA
sequences have been submitted to GenBank, as detailed in Table 1.
A naturally occurring bacterial strain with > 99% 16S rDNA
sequence similarity to strain PM1 was detected in groundwater collected at various locations at Port Hueneme, including outside the plots where the organism was inoculated.
Suspected novel species will be confirmed by 16S rDNA
Bacteria were also grown on nitrogen-free marine media and identified by sequencing the small subunit 16s rDNA
The copy number of P aches 16S rDNA
present in the surgical specimen of the current case was approximately 37-fold the levels in control specimen.