an industry leader in comprehensive metagenomic services and data analysis, has provided 16S Gene
Sequencing Services, including library preparation, sequencing and bioinformatics, to DNA Genotek for the validation of its OMNIgene[sup.
We have already amplified the 16S gene
using standard extraction and PCR techniques.
To determine if particular bacterial species correlate with rheumatoid arthritis, the researchers sequenced the so-called 16S gene
on 44 fecal DNA samples from newly diagnosed patients with rheumatoid arthritis prior to immune-suppressive treatment; 26 samples from patients with chronic, treated rheumatoid arthritis; 16 samples from patients with psoriatic arthritis (characterized by red, flaky skin in conjunction with joint inflammation); and 28 samples from healthy individuals.
Amplification of the 16S gene
was based on the following cycling parameters: 2 min at 94[degrees]C for denaturation, followed by 30 cycles of 30 sec at 94[degrees]C, 1 min at 51 [degrees]C for annealing, and 2 min at 72[degrees]C for extension, and then 7 min at 72[degrees]C for the final extension.
data) were used to amplify a 500-bp fragment of the 16S gene
of Anaplasma/Ehrlichia spp.
Genetic variation of Reticulitermes flavipes (Isoptera: Rhinotermitidae) in North America Applying the mitochondrial rRNA 16S Gene
More than 170 zebra mussel bacterial 16S gene
sequences were checked for similarity to 16S sequences contained in the Ribosomal Database Project and BLAST public databases.