We have already amplified the 16S gene
using standard extraction and PCR techniques.
The researchers sequenced the 16S gene
on fecal samples of 114 people, including patients newly diagnosed with rheumatoid arthritis, prior to their treatment; patients with chronic, but treated, RA; patients with psoriatic arthritis; and healthy individuals.
To determine if particular bacterial species correlate with rheumatoid arthritis, the researchers sequenced the so-called 16S gene
on 44 fecal DNA samples from newly diagnosed patients with rheumatoid arthritis prior to immune-suppressive treatment; 26 samples from patients with chronic, treated rheumatoid arthritis; 16 samples from patients with psoriatic arthritis (characterized by red, flaky skin in conjunction with joint inflammation); and 28 samples from healthy individuals.
The 16S gene
is being studied using an Ion Torrent Personal Genome Machine, which can identify ten million bacteria in just three hours.
Amplification of the 16S gene
was based on the following cycling parameters: 2 min at 94[degrees]C for denaturation, followed by 30 cycles of 30 sec at 94[degrees]C, 1 min at 51 [degrees]C for annealing, and 2 min at 72[degrees]C for extension, and then 7 min at 72[degrees]C for the final extension.
We amplified and sequenced a 550-bp region of the 16S gene
using standard amphibian primers 16SA-L and 16SB-H (Vences and others 2005).
rDNA 16S gene
amplification from Klebsiella genus species were performed under the following reaction conditions: Klebrib-1 (5'-GTAATGT CTGGGAAACTGCC-3') [0.
data) were used to amplify a 500-bp fragment of the 16S gene
of Anaplasma/Ehrlichia spp.
Genetic variation of Reticulitermes flavipes (Isoptera: Rhinotermitidae) in North America Applying the mitochondrial rRNA 16S Gene
More than 170 zebra mussel bacterial 16S gene
sequences were checked for similarity to 16S sequences contained in the Ribosomal Database Project and BLAST public databases.
pneumoniae rhinoscleromatis was tested in silico through gene pattern prediction of 759 bp and 311 bp of a sequence rDNA 16S gene
with Sal I restriction enzyme (Figure 2A); nevertheless, due to polymorphism described among the ADNr 16S gene
Amplification primers 5'-CCTAACACATGCAAGTCGARCG-3' (forward) and 5'-CGTAT-TACCGCGGCTGCT-3' (reverse), both from Eurogentec (Seraing, Belgium) were used in a standard polymerase chain reaction (PCR) to generate a 490-bp fragment from the 5' end of the 16S gene