polyacrylamide gel

(redirected from Acrylamide gels)
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polyacrylamide gel

n.
A hydrated polymer consisting of a long chain of amide groups, used as a medium for substances that undergo gel electrophoresis.
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References in periodicals archive ?
A mixture containing 10 ^L of venom sample (2 mg/ml of crude venom or 1 mg/ml of HPLC fractions), 5 [micro]l of 2X sample buffer (Novex, Invitrogen) and 1 [micro]l of 10X sample reducing agent NUPAGE (Invitrogen[TM], USA) was heated to 100 [grados]C for 5 min and loaded on to a10-20% Tricine acrylamide gels (Invitrogen[TM], USA).
Sample preparation and loading: Add an aliquot (5 l) of 10A-loading buffer to the completed PCR reactions in tube mix well and load 5 l sample to the agarose gels and load the acrylamide gels with a sample volume of 3 l.
The acrylamide gels were stained by means of the silver staining protocol described by Creste et al., [9] PCR bands up-regulated by aluminum stress were chosen and sent for sequencing.
Magill said acrylamide gels are the most widely used chemical grout for lateral repairs.
Single percentage acrylamide gels will favor the separation of proteins in a narrow pH range.
In addition, the small size fragments produced after restriction enzyme digestion are usually resolved by acrylamide gels electrophoresis, thus limiting the number of samples that can be examined at one time.
A rinse solution is for rinsing the plate off, and Acryl-Glide Wipes prevent the glass plates from sticking to the acrylamide gels. The kit contains sufficient materials to prepare 10 slab gel sequencing plate sets or 20-40 mini gel plate sets and can be stored at room temperature.
Initial concentrations of the compounds in acrylamide gels were 1 mg/ml (fluorescein) and 2 X [10.sup.-4] M (GGR).
During the gel drying of the acrylamide gels, there was no visible condensation at the exhaust port of the chemical scroll pump.
This allows accurate quantitative analysis of large numbers of RPA samples while avoiding the hazards of acrylamide gels.
uniform concentration of acrylamide gels for broad pH range.
The nondenaturing electrophoresis system has nearly the same resolution as that obtained with denaturing acrylamide gels and silver staining.