To assess the efficacy of these vectors in the [NiV.sub.B] African green monkey
model, 4 groups of African green monkeys
received a single intramuscular vaccination of rVSV vectors on day -28 (Figure 2).
Chlorocebus aethiops, formerly known as Cercopithecus aethiops, is commonly referred to as the African green monkey
, vervet, tantulus, savannah, or the grivet monkey.
Several cell lines, such as African green monkey
kidney (Vero, CV-1), baby hamster kidney (BHK), fetal rhesus monkey kidney (FRhK), human embryonic lung (HEL), human fetal foreskin (ff), mink lung (ML), primary rabbit kidney (PRK), permanent rabbit kidney (RK13), and HeLa cell line have been used for HSV propagation (12,13).
A scanning electron micrograph of the Ebola virus budding from the surface of a Vero cell of an African green monkey
kidney epithelial cell line.
Moreover, they succeeded in culturing viruses from two of the camels in "vero" (African green monkey
) cells in their lab.
A linhagem celular VERO (African Green monkey
kidney) e a mais utilizada para a propagacao do agente in vitro (DUBEY, 2003).
But now a line of African green monkey
kidney cells known as Vero cells has been cleared for use in reverse genetics.
The rabbit and monkey antisera against the synthetic peptides were diluted to 1:40-fold with PBS, and 10 [micro]L of each diluted serum was added to a well of the slide that was coated with SARS-CoV-infected African green monkey
kidney Vero cells and incubated at 37 [degrees]C for 1 h.
(Columbia, MD; 301-854-3900) announced it has been granted United States Patent 6,025,182 "Method for Producing a Virus from an African Green Monkey
Kidney Cell Line" developed by Louis Potash, PhD, director of the Novavax biomedical services division.
In each case, phylogenetic analyses indicated that the viruses were closely related to SIVAGM from the local species of African green monkey
, namely, vervet monkeys in East Africa and sabaeus monkeys in West Africa [ILLUSTRATION FOR FIGURE 1 OMITTED].
Genetic tests indicate that the viruses came from chimpanzees, baboons, and an African green monkey
subtilis results are similar to the minimal effects seen when Vero cells (from kidney of African green monkey
) were treated 2 hr with culture supernatants from B.