Alleles were labeled alphabetically, in the decreasing electrophoretic mobility order of their corresponding allozymes
Here, an investigation based on wider sampling in terms of space and time, and a combined analysis of allozymes
, DNA markers and morphometries are presented.
Isoenzymes may have different allelic forms (allozymes
), mostly selective neutrals and co-dominants, which could be used to quantify allelic frequencies and different measures of genetic variation.
The founder effect on genetic variability of experimental populations of mosquito fish were measured by the allozymes
electrophoretic technique by Leberg (1992), who estimated the values of proportion of polymorphic loci, number of alleles per locus and heterozygosity.
, the genetic distance indexes resulted in a low to moderate differentiation between PMA and SSF (Nei's D = 0.0248; Fst = 0.0556).
These authors based this idea from the zero genetic diversity found in the populations studied with allozymes
Staining pattern and genetic interpretation of band patterns of allozymes
followed standard principles of Wendel and Weeden (1989).
The genus is about 15 million years old (Bolnick et al., 2006) and given its wide distribution and abundance has been subjected to a series of evolutionary investigations using several markers, including: allozymes
, external morphology, osteology, mitochondrial DNA, and nuclear DNA (Wainwright and Lauder, 1992; Mabee, 1993; Near et al., 2004; Harris et al., 2005; Bolnick et al., 2006).
have been used for a number of years to describe chum salmon population differentiation and structure (Okazaki, 1982a; Kijima and Fujio, 1982; Wilmot et al., 1994; Efremov, 2001; Salmenkova et al., 2007).
.--Parts of the legs were homogenized in distilled water for performing the cellulose acetate gel electrophoresis, following the procedures of Hebert & Beaton (1989).
As far as the analysis of the allozymes
is concerned, seven (GLUDH*, LDH-1*, LDH-2*, LDH-3*, MDH-1*, PROT-1*, PROT-2*) out of the 20 presumptive loci investigated were monomorphic in both species.
have been detected for many mosquitoes and in some cases the isozyme characters appear to be correlated with vector status or another biological attribute.