Immobiline DryStrips (length, 18 cm; GE Healthcare, UK), containing immobilized ampholytes
forming a linear pH gradient of 4 to 7, were used for isoelectric focusing (IEF) in the first dimension.
a pioneer in designing and manufacturing the most reliable, high performance, and high throughput CEInfinite whole column imaging detection capillary isoelectric focusing (iCIEF) systems, proprietary cartridges, ampholytes
, and pI standards for the life science industry, is pleased to announce Isogen Life Science B.
4% (v/v) carrier ampholytes
(pH 3-10, BioRad, USA) and 50 L of protease inhibitor cocktail (Catalogue No.
Analytical method: For sample processing, was used the technique of the isoelectric focusing electrophoresis (IEF), in which the separation of hemoglobin was performed by loading a blood sample on an agarose gel containing ampholytes
with a pH between 6 and 8 and quantified by densitometry.
The isoelectric focusing (IEF) was performed without ampholytes
and glycerol according to the manufacturer's instructions for peptide focusing.
It was established that synthesized ampholytes
possess sufficient high sorption and complexing ability in relation to ions of copper, nickel, uranyl both from sour and alkaline solutions.
Although gel-based techniques for protein separation are tremendously powerful, the observation of protein bands reflects a complex set of interactions between protein, ampholytes
, buffer composition, gel composition, and pore size.
5M Urea, 4% NP-40, 5% (v/v) 2-mercaptoethanol, 2% v/v ampholytes
of pH 5-8 and pH 3-10.
The isoelectric focusing of proteins can be determined using immobilized pH gradients or ampholytes
were removed by bringing each fraction to 1 M NaCl for 15 min and then aliquots of 10-20 [micro]L of each fraction were used for protein analysis.
5:1, 9 M urea, 2% ampholytes
(pH range 3-10), and 1% Triton X-100.