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Related to Ampholytes: isoelectric point, zwitterion


 (zwĭt′ər-ī′ən, swĭt′-, tsvĭt′-)
A molecule, especially an amino acid, containing a positively charged ion at one end and a negatively charged ion at the other.

[German : Zwitter, hybrid (from Middle High German zwitarn, from Old High German, from zwi-, twice; see dwo- in Indo-European roots) + Ion, ion (from Greek; see ion).]

zwit′ter·i·on′ic (-ī-ŏn′ĭk) adj.
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.


(Chemistry) chem an ion that carries both a positive and a negative charge
[C20: from German Zwitter hermaphrodite + ion]
zwitterionic adj
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014


(ˈzwɪt ərˌaɪ ən, ˈswɪt-, ˈtsvɪt-)

an ion with both a positive and a negative charge.
[< German Zwitterion (1897) =Zwitter hybrid, hermaphrodite + Ion ion]
Random House Kernerman Webster's College Dictionary, © 2010 K Dictionaries Ltd. Copyright 2005, 1997, 1991 by Random House, Inc. All rights reserved.
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Five IPG Immobiline DryStrips 24 cm, pH 3 to 10 (GE Healthcare, Bucks, United Kingdom) were rehydrated overnight in 450 [micro]l 2DE rehydration buffer (7M urea, 2M thiourea, 2% CHAPS, 0.5% pH 3 to 11 ampholytes (GE Healthcare), 1% DTT, trace bromophenol blue).
Protein samples (1000 mg of total soluble protein) were diluted in 350 mL Destreak rehydration solution (GE Healthcare), supplemented with 0.2% ampholytes 3-10, and applied to IPG strips (pH 3-10 NL -17 cm, Bio-Rad, USA) by in-gel rehydration.
For each sample, 350 [micro]g of venom proteins homogenized in rehydration buffer (7 molx[L.sup.-1] urea, 2 molx[L.sup.-1] thiourea, 1% CHAPS, and traces of bromophenol blue) have been used with DTT 40 mmolx[L.sup.-1] and 0.5% ampholytes at pH 3-10NL with a final volume of 250 [micro]L.
For two-dimensional electrophoresis (2D) the IPG strips (17 cm and pH 4-7) were passively rehydrated during 16 h with 300 [micro]L hydration buffer (8M urea, 2% CHAPS, 50mM DTT, 0,2% ampholytes 3-10, and 0,001% bromophenol blue) containing 10 [micro]g of the high abundance proteins or 15 [micro]g of the low abundance proteins.
Immobiline DryStrips (length, 18 cm; GE Healthcare, UK), containing immobilized ampholytes forming a linear pH gradient of 4 to 7, were used for isoelectric focusing (IEF) in the first dimension.
The separation of different haemoglobin (F, A, S, and other types of haemoglobin) was obtained after application on thin layer home-made agarose gel containing ampholytes pH 6-8 (ref.
Samples were further adjusted for IEF fractionation by combining 900 [micro]L pooled sample with ampholytes (150 [micro]L, pH 3-10; Novex, Thermo Fisher, ZM0021; Waltham, MA), dithiolthreitol (DTT; 25 [micro]L, 4 M), and bromphenol blue (20 [micro]L, 10mg/mL).
The isoelectric pH gradient (IPG) tube gel was prepared using 8 M urea; 4% acrylamide; 2.4% ampholytes; 2.4% CHAPS; and NP40 according to Protean IEF Cell (Bio-Rad, USA) manufacturer's instructions.
Cambridge, Canada, February 11, 2016 --( AES (Advanced Electrophoresis Solutions Ltd.), a pioneer in designing and manufacturing the most reliable, high performance, and high throughput CEInfinite whole column imaging detection capillary isoelectric focusing (iCIEF) systems, proprietary cartridges, ampholytes, and pI standards for the life science industry, is pleased to announce Isogen Life Science B.V.
Extraction of proteins: Approximately 1 g of pulverised muscle tissue was homogenised (Wiggen Hauser D500, USA) in 3 mL of ice cold extraction buffer containing 7M Urea, 2 M Thiourea, 50 mM DTT (dithiothreitol), 4% (w/v) 3-[(3-cholamidopropyl) dimethylammonio]-1- propanesulfonate (CHAPS), 0.4% (v/v) carrier ampholytes (pH 3-10, BioRad, USA) and 50 L of protease inhibitor cocktail (Catalogue No.
Analytical method: For sample processing, was used the technique of the isoelectric focusing electrophoresis (IEF), in which the separation of hemoglobin was performed by loading a blood sample on an agarose gel containing ampholytes with a pH between 6 and 8 and quantified by densitometry.
The isoelectric focusing (IEF) was performed without ampholytes and glycerol according to the manufacturer's instructions for peptide focusing.